regulation of small rna accumulation in the maize shoot apex监管在玉米苗端小rna的积累.pdfVIP

Regulation of Small RNA Accumulation in the Maize Shoot Apex 1. 1,2. 1 3 3 Fabio T. S. Nogueira , Daniel H. Chitwood , Shahinez Madi , Kazuhiro Ohtsu , Patrick S. Schnable , Michael J. Scanlon4, Marja C. P. Timmermans1,2* 1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America, 2 Watson School of Biological Sciences, Cold Spring Harbor, New York, United States of America, 3 Center for Plant Genomics, Roy J. Carver Co-Laboratory, Iowa State University, Ames, Iowa, United States of America, 4 Department of Plant Biology, Cornell University, Ithaca, New York, United States of America Abstract MicroRNAs (miRNAs) and trans-acting siRNAs (ta-siRNAs) are essential to the establishment of adaxial–abaxial (dorsoventral) leaf polarity. Tas3-derived ta-siRNAs define the adaxial side of the leaf by restricting the expression domain of miRNA miR166, which in turn demarcates the abaxial side of leaves by restricting the expression of adaxial determinants. To investigate the regulatory mechanisms that allow for the precise spatiotemporal accumulation of these polarizing small RNAs, we used laser-microdissection coupled to RT-PCR to determine the expression profiles of their precursor transcripts within the maize shoot apex. Our data reveal that the pattern of mature miR166 accumulation results, in part, from intricate transcriptional regulation of its precursor loci and that only a subset of mir166 family members contribute to the establishment of leaf polarity. We show that miR390, an upstream determinant in leaf polarity whose activity triggers tas3 ta-siRNA biogenesis, accumulates adaxially in leaves.