research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride研究兔干眼模型的稳定性引起的局部应用防腐剂苯扎氯铵.pdfVIP

Research on the Stability of a Rabbit Dry Eye Model Induced by Topical Application of the Preservative Benzalkonium Chloride 1. 2. 1 1,2 1 3 3 Chaoyang Li , Yiyue Song , Shaohong Luan , Pengxia Wan , Naiyang Li , Jing Tang , Yu Han , Cuiju Xiong1, Zhichong Wang1,2* 1 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China, 2 Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China, 3 Department of Ophthalmology, People’s Hospital of Leshan, Leshan, China Abstract Background: Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium chloride (BAC) and determined the valid time of this model. Methods and Findings: Eighty white rabbits were randomly divided into four groups. One eye from each rabbit was randomly chosen to receive topical 0.1% BAC twice daily for 2 weeks (Group BAC-W2), 3 weeks (Group BAC-W3), 4 weeks (Group BAC-W4), or 5 weeks (Group BAC-W5). Fluorescein staining, Schirmer’s tests, and conjunctival impression cytology were performed before BAC treatment (normal) and on days 0, 7, 14 and 21 after BAC removal. The eyeballs were collected at these time points for immunofluorescence staining, hematoxylin and eosin (HE) staining, and electron microscopy. After removing BAC, the signs of dry eye in Group BAC-W2 lasted one week. Compared with normal, there were still significant differences in the results of Schirmer’s