原创力文档resistance to bmnpv via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, bombyx moribmnpv阻力通过超表达的外源基因控制一个诱导启动子和增强器在转基因蚕,家蚕.pdfVIP
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resistance to bmnpv via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, bombyx moribmnpv阻力通过超表达的外源基因控制一个诱导启动子和增强器在转基因蚕,家蚕
Resistance to BmNPV via Overexpression of an
Exogenous Gene Controlled by an Inducible Promoter
and Enhancer in Transgenic Silkworm, Bombyx mori
1 1 1 2 1 1 1
Liang Jiang , Tingcai Cheng , Ping Zhao , Qiong Yang , Genhong Wang , Shengkai Jin , Ping Lin ,
2 1
Yang Xiao , Qingyou Xia *
1 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, P. R. China, 2 Sericulture and Farm Product Processing Research Institute,
Guangdong Academy of Agricultural Sciences, Guangzhou, China
Abstract
The hycu-ep32 gene of Hyphantria cunea NPV can inhibit Bombyx mori nucleopolyhedrovirus (BmNPV) multiplication in co-
infected cells, but it is not known whether the overexpression of the hycu-ep32 gene has an antiviral effect in the silkworm,
Bombyx mori. Thus, we constructed four transgenic vectors, which were under the control of the 39 K promoter of BmNPV
(39 KP), Bombyx mori A4 promoter (A4P), hr3 enhancer of BmNPV combined with 39 KP, and hr3 combined with A4P.
Transgenic lines were created via embryo microinjection using practical diapause silkworm. qPCR revealed that the
expression level of hycu-ep32 could be induced effectively after BmNPV infection in transgenic lines where hycu-ep32 was
controlled by hr3 combined with 39 KP (i.e., HEKG). After oral inoculation of BmNPV with 3 6105 occlusion bodies per third
instar, the mortality with HEKG-B was approximately 30% lower compared with the non-transgenic line. The economic
characteristics of the transgenic lines remained unchanged. These results suggest that overexpression of an exogenous
antiviral gene controlled by an inducible promoter and en
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