rhodococcus equi’s extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes红球菌属等的极端抵抗过氧化氢主要是赋予它的一个四过氧化氢酶基因.pdfVIP

Rhodococcus equi ’s Extreme Resistance to Hydrogen Peroxide Is Mainly Conferred by One of Its Four Catalase Genes 1. ´ 1. 1¤a ´ 1¤b 2 Pauline Bidaud , Laurent Hebert , Corinne Barbey , Anne-Cecile Appourchaux , Riccardo Torelli , 2 1 1 Maurizio Sanguinetti , Claire Laugier , Sandrine Petry * ´ 1 Dozule Laboratory for Equine Diseases, Unit Bacteriology and Parasitology, ANSES, Goustranville, France, 2 Institute of Microbiology, Catholic University of Sacred Heart, Rome, Italy Abstract Rhodococcus equi is one of the most widespread causes of disease in foals aged from 1 to 6 months. R. equi possesses antioxidant defense mechanisms to protect it from reactive oxygen metabolites such as hydrogen peroxide (H O ) 2 2 generated during the respiratory burst of phagocytic cells. These defense mechanisms include enzymes such as catalase, which detoxify hydrogen peroxide. Recently, an analysis of the R. equi 103 genome sequence revealed the presence of four potential catalase genes. We first constructed DkatA-, DkatB-, DkatC-and DkatD-deficient mutants to study the ability of R. equi to survive exposure to H2O2 in vitro and within mouse peritoneal macrophages. Results showed that DkatA and, to a lesser extent DkatC, were affected by 80 mM H O . Moreover, katA deletion seems to significantly affect the ability of R. equi