原创力文档ring-like distribution of constitutive heterochromatin in bovine senescent cells环状结构异染色质在牛衰老细胞的分布.pdfVIP
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ring-like distribution of constitutive heterochromatin in bovine senescent cells环状结构异染色质在牛衰老细胞的分布
Ring-Like Distribution of Constitutive Heterochromatin
in Bovine Senescent Cells
1,2,3 1,2 1,2 3
Andrey Pichugin *, Nathalie Beaujean , Xavier Vignon , Yegor Vassetzky *
´ ´
1 INRA UMR 1198 Groupe Biologie du Developpement et Reproduction, Jouy en Josas, France, 2 ENVA UMR 1198 Groupe Biologie du Developpement et Reproduction,
´ ´
Jouy en Josas, France, 3 Centre National de la Recherche Scientifique UMR 8126, Universite Paris-Sud 11, Institut de Cancerologie Gustave-Roussy, Villejuif, France
Abstract
Background: Cells that reach ‘‘Hayflick limit’’ of proliferation, known as senescent cells, possess a particular type of nuclear
architecture. Human senescent cells are characterized by the presence of highly condensed senescent associated
heterochromatin foci (SAHF) that can be detected both by immunostaining for histone H3 three-methylated at lysine 9
(H3K9me3) and by DAPI counterstaining.
Methods: We have studied nuclear architecture in bovine senescent cells using a combination of immunofluorescence and
3D fluorescent in-situ hybridization (FISH).
Results: Analysis of heterochromatin distribution in bovine senescent cells using fluorescent in situ hybridization for
pericentric chromosomal regions, immunostaining of H3K9me3, centromeric proteins CENP A/B and DNA methylation
showed a lower level of heterochromatin condensation as compared to young cells. No SAHF foci were observed. Instead,
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