role of quantiferon-tb gold, interferon gamma inducible protein-10 and tuberculin skin test in active tuberculosis diagnosisquantiferon-tb黄金的作用,诱导干扰素γprotein-10和结核菌素皮肤试验诊断活动性结核病.pdfVIP

Role of QuantiFERON-TB Gold, Interferon Gamma Inducible Protein-10 and Tuberculin Skin Test in Active Tuberculosis Diagnosis 1 2 2 3 Basirudeen Syed Ahamed Kabeer , Balambal Raman , Aleyamma Thomas , Venkatesan Perumal , Alamelu Raja1* 1 Department of Immunology, Tuberculosis Research Centre (ICMR), Chennai, Tamil Nadu, India, 2 Department of Clinic, Tuberculosis Research Centre (ICMR), Chennai, Tamil Nadu, India, 3 Department of Statistics, Tuberculosis Research Centre (ICMR), Chennai, Tamil Nadu, India Abstract Background: The measurement of Interferon gamma or Interferon gamma inducible protein (IP)-10 in antigen stimulated blood samples is suggested as an alternative method for latent tuberculosis (TB) diagnosis. Nonetheless, their role in active TB diagnosis, particularly in TB endemic settings is yet to be defined. In this study, the sensitivities and specificities of Interferon gamma release assay (IGRA), IP-10 assay and tuberculin skin test (TST) in detecting active TB cases were assessed in human immunodeficiency virus (HIV) sero-negative TB patients and healthy controls respectively. Methods/Principal Findings: A total of 177 adult TB patients and 100 healthy controls were included for this study. QuantiFERON-TB Gold In-tube (QFT-IT) method was used to analyze the sensitivity and specificity of IGRA. QFT-IT, IP-10 and TST yielded the diagnostic sensitivities of 90.6% (95%CI: 86.3%–94.9%), 92.5% (95%CI: 88.6%–96.4%) and 68.9% (95%CI: 60.6%–77.2%) and specificities of 55% (95% CI: 35.2%–54.8%), 48% (95% CI: 38.2%–57.8%) and 75.5% (95% CI: 66.8%– 84.2%), respectively. The extent of pulmonary involvement or presence of diabetes mellitus did not appear to influence the sen