rsf governs silent chromatin formation via histone h2av replacementrsf支配沉默染色质形成通过组蛋白h2av替换.pdfVIP

RSF Governs Silent Chromatin Formation via Histone H2Av Replacement 1,2 1¤ 2 3 1 Kazuma Hanai , Hirofumi Furuhashi , Takashi Yamamoto , Koji Akasaka , Susumu Hirose * 1 Department of Developmental Genetics, National Institute of Genetics, Shizuoka-ken, Japan, 2 Department of Mathematical and Life Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan, 3 Misaki Marine Biological Station, Graduate School of Science, The University of Tokyo, Miura, Kanagawa, Japan Abstract Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation. Citation: Hanai K, Furuhashi H, Yamamoto T, Akasaka K, Hirose S (2008) RSF Governs Silent Chromatin Formation via Histone H2Av Replacement. PLoS Genet 4(2): e1000011. doi:10.1371/journal.pgen.1000011 Editor: Asifa Akhtar, European Molecular Bio