scavenger receptor cd36 expression contributes to adipose tissue inflammation and cell death in diet-induced obesity清道夫受体cd36的表达导致食源性肥胖的脂肪组织炎症和细胞死亡.pdfVIP

Scavenger Receptor CD36 Expression Contributes to Adipose Tissue Inflammation and Cell Death in Diet- Induced Obesity 1,2 . 2,4. 1,2 3 1,2,3 Lei Cai * , Zhen Wang , Ailing Ji , Jason M. Meyer , Deneys R. van der Westhuyzen 1 Department of Veterans Affairs Medical Center, Lexington, Kentucky, United States of America, 2 Department of Internal Medicine, Cardiovascular Research Center, Graduate Center for Nutritional Sciences, University of Kentucky, Lexington, Kentucky, United States of America, 3 Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, United States of America, 4 Department of Physiology, Shandong University School of Medicine, Jinan, People’s Republic of China Abstract Objective: The enlarged adipose tissue in obesity is characterized by inflammation, including the recruitment and infiltration of macrophages and lymphocytes. The objective of this study was to investigate the role of the scavenger receptor CD36 in high fat diet-induced obesity and adipose tissue inflammation and cell death. Experimental Approach: Obesity and adipose tissue inflammation was compared in CD36 deficient (CD36 KO) mice and wild type (WT) mice fed a high fat diet (60% kcal fat) for 16 weeks and the inflammatory response was studied in primary adipocytes and macrophages isolated from CD36 KO and WT mice. Results: Compared to WT mice, CD36 KO mice fed a high fat diet exhibited reduced adiposity and adipose tissue inflammation, with decreased adipocyte cell death, pro-inflammatory cytokine expression and macrophage and T-cell accumulation. In primary cell culture, the absence of CD36 exp