sds can be utilized as an amyloid inducer a case study on diverse proteins可以利用sds作为淀粉样蛋白诱导一个案例研究在不同的蛋白质.pdfVIP

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sds can be utilized as an amyloid inducer a case study on diverse proteins可以利用sds作为淀粉样蛋白诱导一个案例研究在不同的蛋白质.pdf

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sds can be utilized as an amyloid inducer a case study on diverse proteins可以利用sds作为淀粉样蛋白诱导一个案例研究在不同的蛋白质

SDS Can Be Utilized as an Amyloid Inducer: A Case Study on Diverse Proteins 1 1 1 1 Javed Masood Khan , Atiyatul Qadeer , Sumit Kumar Chaturvedi , Ejaz Ahmad , Syed Arif Abdul 2 2 1 Rehman , Samudrala Gourinath , Rizwan Hasan Khan * 1 Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India, 2 School of Life Sciences, Jawaharlal Nehru University, New Delhi, India Abstract Sodium dodecyl sulphate (SDS), an anionic surfactant that mimics some characteristics of biological membrane has also been found to induce aggregation in proteins. The present study was carried out on 25 diverse proteins using circular dichroism, fluorescence spectroscopy, dye binding assay and electron microscopy. It was found that an appropriate molar ratio of protein to SDS readily induced amyloid formation in all proteins at a pH below two units of their respective isoelectric points (pI) while no aggregation was observed at a pH above two units of pI. We also observed that electrostatic interactions play a leading role in the induction of amyloid. This study can be used to design or hypothesize a molecule or drug, which may counter act the factor responsible for amyloid formation. Citation: Khan JM, Qadeer A, Chaturvedi SK, Ahmad E, Abdul Rehman SA, et al. (2012) SDS Can Be Utilized as an Amyloid Inducer: A Case Study on Diverse Proteins. PLoS ONE 7(1): e29694. doi:10.1371/journal.pone.0029694 Editor: Vladimir N. Uversky, University of South Florida College of Medicine, United States of America Received September 23, 2011; Accepted December 2, 2011; Published January 12, 2012 Copyright: 2012 Khan et al. This is an open-access article distri