sepsid even-skipped enhancers are functionally conserved in drosophila despite lack of sequence conservationsepsid甚至没有增强剂是在果蝇尽管缺乏序列功能守恒的.pdfVIP

Sepsid even-skipped Enhancers Are Functionally Conserved in Drosophila Despite Lack of Sequence Conservation 1. 1,2. 1 3 1,2,4,5 Emily E. Hare , Brant K. Peterson , Venky N. Iyer , Rudolf Meier , Michael B. Eisen * 1 Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, United States of America, 2 Center for Integrative Genomics, University of California Berkeley, Berkeley, California, United States of America, 3 Department of Biological Sciences, National University of Singapore, Singapore, 4 Genomics Division, Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, 5 California Institute for Quantitative Biosciences, Berkeley, California, United States of America Abstract The gene expression pattern specified by an animal regulatory sequence is generally viewed as arising from the particular arrangement of transcription factor binding sites it contains. However, we demonstrate here that regulatory sequences whose binding sites have been almost completely rearranged can still produce identical outputs. We sequenced the even- skipped locus from six species of scavenger flies (Sepsidae) that are highly diverged from the model species Drosophila melanogaster, but share its basic patterns of developmental gene expression. Although there is little sequence similarity between the sepsid eve enhancers and their well-characterized D. melanogaster counterparts, the sepsid and Drosophila enhancers drive nearly identical expression patterns in transgenic D. melanogaster embryos. We conclude that the molecular machinery that connects regulatory sequences to the transcription apparatus