serum microrna signatures identified by solexa sequencing predict sepsis patients’ mortality a prospective observational study血清microrna签名由solexa测序确定预测脓毒症患者的死亡率前瞻性观察性研究.pdfVIP

Serum MicroRNA Signatures Identified by Solexa Sequencing Predict Sepsis Patients’ Mortality: A Prospective Observational Study 1,2,3. 2,4. 5 6 3 1,3 Huijuan Wang , Pengjun Zhang , Weijun Chen , Dan Feng , Yanhong Jia , Lixin Xie * 1 Department of Respiratory Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya, Hainan Province, China, 2 Medical College, Nankai University, Tianjin, People’s Republic of China, 3 Department of Respiratory Medicine, Chinese PLA General Hospital, Beijing, China, 4 Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China, 5 Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Airport Industry B6, Beijing, China, 6 Department of Medical Statistics, Chinese PLA General Hospital, Beijing, China Abstract Background: Sepsis is the leading cause of death in Intensive Care Units. Novel sepsis biomarkers and targets for treatment are needed to improve mortality from sepsis. MicroRNAs (miRNAs) have recently been used as finger prints for sepsis, and our goal in this prospective study was to investigate if serum miRNAs identified in genome-wide scans could predict sepsis mortality. Methodology/Principal Findings: We enrolled 214 sepsis patients (117 survivors and 97 non-survivors based on 28-day mortality). Solexa sequencing followed by quantitative reverse transcriptase polymerase chain reaction assays was used to test for differences in the levels of miRNAs between survivors and non-survivors. miR-223, miR-15a, miR-16, miR-122, miR- 193*, and miR-483-5p were significantly differentially expressed. Receiver operating characteristic curves were generated