serum metabolomics reveals higher levels of polyunsaturated fatty acids in lepromatous leprosy potential markers for susceptibility and pathogenesis血清代谢组学揭示了更高水平的多不饱和脂肪酸在麻风结节的麻风病的潜在标记物磁化率和发病机理.pdfVIP

Serum Metabolomics Reveals Higher Levels of Polyunsaturated Fatty Acids in Lepromatous Leprosy: Potential Markers for Susceptibility and Pathogenesis 1 1 2 3 1 Reem Al-Mubarak , Jason Vander Heiden , Corey D. Broeckling , Marivic Balagon , Patrick J. Brennan , Varalakshmi D. Vissa1* 1 Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America, 2 Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, Colorado, United States of America, 3 Leonard Wood Memorial Center for Leprosy Research, Cebu, Philippines Abstract Background: Leprosy is a disease of the skin and peripheral nervous system caused by the obligate intracellular bacterium Mycobacterium leprae. The clinical presentations of leprosy are spectral, with the severity of disease determined by the balance between the cellular and humoral immune response of the host. The exact mechanisms that facilitate disease susceptibility, onset and progression to certain clinical phenotypes are presently unclear. Various studies have examined lipid metabolism in leprosy, but there has been limited work using whole metabolite profiles to distinguish the clinical forms of leprosy. Methodology and Principal Findings: In this study we adopted a metabolomics approach using high mass accuracy ultrahigh pressure liquid chromatography mass spectrometry (UPLC-MS) to investigate the circulatory biomarkers in newly diagnosed untreated leprosy patients. Sera from patients having bacterial indices (BI) below 1 or above 4 were selected, subjected to UPLC-MS, and then analyzed for biomarkers which distinguish the polar presentations of leprosy. We