单克隆抗体的制备（Preparation of monoclonal antibodies）.docVIP

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单克隆抗体的制备（Preparation of monoclonal antibodies）.doc

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单克隆抗体的制备（Preparation of monoclonal antibodies）

单克隆抗体的制备（Preparation of monoclonal antibodies） Preparation of monoclonal antibodies.Txt Preparation of monoclonal antibodies In 1975 Kohler and Milstein first reported by cell hybridization by sheep red blood cell (SRBC) mice immunized with murine spleen cells and myeloma cell fusion, established the first B cell hybridoma cell lines, and successfully prepared anti SRBC monoclonal antibodies (monoclonalantibody, McAb). So far, thousands of McAb have been developed worldwide. Monoclonal antibodies have high physical and chemical properties, single biological activity, strong specificity of antigen binding, easy manipulation and quality control, and are easy to source. Therefore, they have been welcomed and valued. In the field of medicine, McAb plays an important role in the diagnosis of disease, prognosis, prevention and treatment of disease and mechanism of disease. To this end, two inventors in 1984 by the Nobel pit?? thin? Section 1 the basic principle of hybridoma technique The basic principle of hybridoma antibody technology is to combine the two cells while maintaining the primary characteristics of both. These two kinds of cells are antigen - mediated mouse spleen cells and mouse myeloma cells. The main features of splenic lymphocytes is its antibody secretion and can be cultured in the media in the selection (selection principle below), mouse myeloma cells can be cultured in condition of infinite division and proliferation, namely?? health Ba Lang? T Ren. Shoot bath =??, Yong Meng long wave twin school? To pry the slips? By squid in? This brain and to chant? in the car with harmonic? What ethics? The weft? He tied the Quan Chen solid Xiong Tong 3 room to 8?? Guangdong. They went to the South Street hemorrhoids? Si F a non unitary aluspan father? The job street? Can? Ao? (1) cell selection and fusion The aim of hybridoma technology is to prepare antigen specific monoclonal antibodies, so one side of fusion cell must select B cells which have antigen immunity,