CBIQ对肺泡上皮细胞氧化损伤保护作用.docVIP

CBIQ对肺泡上皮细胞氧化损伤保护作用 　 作者：黄颖，张丙芳，招明高，王晓明，黄晨 【摘要】 目的： 研究CBIQ对肺泡上皮细胞株A549氧化损伤的保护作用. 方法： 应用MTT法检测不同浓度CBIQ对正常及过氧化氢（H2O2）损伤的肺泡上皮细胞株A549的保护作用；DNA Ladder检测细胞凋亡；相差显微镜观察Hoechst染色后细胞凋亡形态的变化. 结果： H2O2损伤后的A549细胞经不同浓度CBIQ处理后，细胞死亡数明显减少. 浓度为1×10-2，1×10-3 mmol/L的CBIQ组平均细胞存活率分别为(76.1±0.8)%，(71.3±1.0)%，较损伤组(46.3±0.9)%明显增高(Plt;0.01)；终浓度为100 μmol/L H2O2及10 μmol/L CBIQ共同干预A549细胞4 h后，可检测到凋亡标志性的DNA梯形带；实验组细胞凋亡率较对照组明显下降(Plt;0.05). 结论： 在一定浓度范围内，CBIQ对氧化损伤的A549细胞生长有浓度依赖性的保护作用，并可抑制氧化损伤的A549细胞发生凋亡. 【关键词】 CBIQ；急性病；肺/损伤；肺泡；上皮细胞 　　【Abstract】AIM: To explore the protective role of 4Chlorobenzo［F］isoquinoline (CBIQ) in oxidatively damaged human lung epithelial cell line A549. METHODS: After cultured with different doses of CBIQ, the normal A549 cells and the oxidatively damaged cells were both tested by MTT assay. Cell apoptosis was detected by DNA Ladder. Cell morphological change was observed by Hoechst staining under a phase contrast microscope. RESULTS: After A549 cells were treated with different concentrations of CBIQ, dosedependent protection was demonstrated. The cell viability rate of the group by 1×10-2 and 1×10-3 mmol/L CBIQ were respectively (76.1±0.8)% and (71.3±1.0)%，which were higher markedly than the rate of the injury group (46.3±0.9)% (Plt;0.01). Four hours after exposure to 100 μmol／L H2O2 and 10 μmol/L CBIQ, A549 cells undertook the apoptosis displayed by the DNA Ladder. The percentage of cell apoptosis was lower in experimental group than in control group (Plt;0.05). CONCLUSION: In a certain concentration range, CBIQ can dosedependently protect human lung epithelial cells from oxidatively damage. The activator of cystic fibrosis transmembrane conductance regulator (CFTR) can inhibit the apoptosis of oxidatively damaged A549 cells. 　　【Keywords】 CBIQ; acute disease; lung/injuries; pulmonary alveoli; epithelial cells 　　0引言 　　急性肺损伤(acute lung injury，ALI)是急性呼吸衰竭的重要病因之一. 氧化应激时产生的大量氧自由基和H2O2作用于肺部时可引起肺泡上皮细胞活性降低，甚至产生ALI. 肺泡上皮细胞Na+，Cl-异常跨膜转运在肺水肿形成中起重要作用［1］. 囊性纤维跨膜电导调节因子(CFTR)是肺泡上皮细胞中一种