Na+，K+-ATP酶β1亚基及肿瘤细胞生长相关性.docVIP

Na+，K+-ATP酶β1亚基及肿瘤细胞生长相关性 　 作者：熊竹娟　林苹　张洁　王修杰　王琪　任婧婧　杨洪亮　王静　吴亚英 【摘要】 　　［目的］ 探讨Na+，K+-ATP酶β1亚基（ATP1B1）与肿瘤细胞增殖分化的关系，以及外源性ATP1B1对肿瘤细胞的影响。［方法］ 采用RT-PCR检测肿瘤细胞株、正常外周血单核细胞中ATP1B1的mRNA水平，以及经脂多糖（LPS）促进细胞增殖、二甲亚砜（DMSO）诱导肿瘤细胞分化后ATP1B1的mRNA水平变化，并观察通过转染技术增加肿瘤细胞中ATP1B1表达后细胞的生长情况。［结果］ 肿瘤细胞株中ATP1B1的mRNA水平明显高于正常单核细胞（P＜0.05），促进细胞增殖后细胞中ATP1B1的mRNA水平增高（P＜0.05），诱导分化后降低（P＜0.05），导入外源性ATP1B1 使肿瘤细胞增殖明显受抑（P＜0.05）。［结论］ Na+，K+-ATP酶β1亚基可以成为反映细胞功能状态特别是肿瘤细胞增殖代谢的重要指标，为肿瘤生物治疗的新策略提供重要的实验依据。 【关键词】 Na+　K+-ATP酶β1亚基　肿瘤细胞　增殖　诱导分化 Abstract: ［Purpose］ To explore the relationship between Na+,K+-ATPase β1-Subunit (ATP1B1) and tumor cell in proliferation and differentiation, as well as the effect of ATP1B1 introduction.［Methods］ The levels of ATP1B1 mRNA were detected by RT-PCR in both of tumor cell lines and normal peripheral blood monocyte, and the changing of ATP1B1 mRNA were analyzed in the proliferation-cells stimulated by LPS and the differentiation-cells induced by DMSO. Moreover, survival of cells transfected with ATP1B1 was observed. ［Results］The expression of ATP1B1 mRNA in tumor cell was significantly higher than that of normal monocyte（P＜0.05）. ATP1B1 mRNA level was enhanced in tumor cell lines and monocytes by LPS stimulation（P＜0.05）, while the level of ATP1B1 mRNA of these cells were decreased by DMSO-induced differentiation（P＜0.05）. The growth of the tumor cells transfected with ATP1B1 was inhibited obviously(P＜0.05）. ［Conclusion］ ATP1B1 can be the index of cell functional status, especially tumor cell proliferation and differentiation. It will become an important experiment foundation for the biotherapy of tumor. Key words: Na+,K+-ATPase β1-subunit; tumor cell; proliferation; differentiation Na+，K+-ATP酶是一种广泛存在于真核生物细胞上的跨膜转运蛋白，主要调节细胞内外Na+、K+的平衡，维持细胞的容积和保证细胞内环境的稳定。Na+，K+-ATP酶可能与肿瘤的转移有关，已有将其作为治疗乳腺癌靶点的研究[1]。有关Na+，K+-ATP酶与细胞功能关系的研究逐渐成为关注热点，目前α亚基的研究较多，而对Na+，K+-ATP酶的调控亚基——β亚基的研究较少。我们检测了多种细胞的Na+，K+-ATP酶基因表达情况，发现正常外周血单核细胞与肿瘤细胞株间的ATP1B1 mRNA表达有着显著的差异性，而细胞增殖和细胞分化后ATP1B1基因表达又有变化，向细胞中导入ATP1B1有着明显的抑制细胞增殖效应