人CD4+T细胞在肠道细菌抗原刺激前后CD25及FOXP3表达.docVIP

人CD4+T细胞在肠道细菌抗原刺激前后CD25及FOXP3表达 　【关键词】 调节性T细胞；CD25；FOXP3；肠杆菌科；抗原 　　Expressions of CD25 and FOXP3 on human CD4+T cells before and after challenged by enteric bacterial antigens 　　【Abstract】 AIM: To investigate expressions of CD25 and FOXP3 on human peripheral blood CD4+ T cells before and after activated by autologous enteric bacterial antigens. METHODS: Human peripheral blood lymphocytes and monocytes were isolated from 15 people. Some lymphocytes were cocultured for 14 d with monocytes which were previously challenged by bacterial antigens from autologous intestine. The CD4, CD25 and FOXP3 were measured by flow cytometry on the freshly isolated and the cocultured lymphocytes. RESULTS: Of the freshly isolated lymphocytes, about 43.4% expressed CD4. In the CD4+T cells, the percentages of CD25+, CD25hi, FOXP3+, CD25+FOXP3+, and CD25hiFOXP3+T cells were 5.8%, 1.9%, 5.2%, 3.3% and 1.6%, respectively. After in vitro challenged by autologous bacterial antigens, the total number of lymphocytes slightly increased (by 21.0%, Plt;0.001 vs unchallenged cells). The percentage of CD4+T cells elevated significantly （67.1% vs 43.4%, Plt;0.001）, while the percentages of CD25+, FOXP3+ and CD25+FOXP3+ T cells did not change significantly. The CD4+CD25+FOXP3+T/CD4+CD25+T or CD4+CD25hiFOXP3+ T/CD4+CD25hi T cell ratio decreased (the former from 59.8% to 52.0%; the latter from 86.3% to 77.9%, both Plt;0.05). CONCLUSION: Both CD25 and CD25hi are not the reliable markers of human regulatory T cells, especially when activated in vitro by bacterial antigens. The activation/proliferation of CD4+CD25+FOXP3- T reactive cells predominates that of CD4+CD25+FOXP3+T regulatory cells in response to the challenge of autologous bacterial antigens. 　　【Keywords】 regulatory T cells; CD25; FOXP3; enteric bacteria; antigen 　　【摘要】 目的： 探讨新鲜分离及自身肠道菌群抗原刺激后的人外周血CD4+T细胞CD25和FOXP3表达的相关性及变化规律. 方法： 分离人的外周血单核细胞和淋巴细胞，单核细胞经自身肠道菌群抗原刺激后与淋巴细胞混合培养，新鲜分离及混合培养14 d的淋巴细胞用三色流式细胞术在单细胞水平同时检测CD4，CD25和FOXP3的表达. 结果： 新鲜分离的淋巴细胞约43.4%表