供者自然杀伤细胞减轻GVHD并提高移植成活率实验探究.docVIP

供者自然杀伤细胞减轻GVHD并提高移植成活率实验探究 　 作者：黄纯兰,刘霆,陆晓茜, 孟文彤 【摘要】 本研究观察激活的供、受者NK细胞能否减轻骨髓移植后GVHD和增加移植成活率。以C57BL/6小鼠作为供者，将接受6.5 Gy 60CO γ射线全身照射的BALB/c受鼠随机分为4组，对照1组（无任何处理）、对照2组（骨髓移植）、实验1组（骨髓移植同时输注激活的受者NK细胞）和实验2组（骨髓移植同时输注激活的供者NK细胞），观察每组小鼠的活存期、嵌合率、GVHD临床和病理改变。结果表明：对照1组全部活存，实验1组比对照2组活存期短（Plt;0.05），实验2组比对照2组活存期延长（Plt;0.01）。实验1 组GVHD临床及病理评分高于对照2组（Plt;0.05），实验2组 GVHD临床及病理评分明显低于对照2组（Plt;0.01）。实验1组和实验2组嵌合率均较对照2组高（Plt;0.05），实验2组的嵌合率比实验1组明显增加（Plt;0.01）。结论：激活的供者NK细胞能延长实验小鼠的活存期，降低GVHD严重程度，增加移植成活率；而激活的受者NK细胞却缩短实验小鼠的活存期，加重GVHD严重程度。受者NK细胞也增加移植成活率，但比供者NK细胞作用小。 【关键词】 骨髓移植;NK细胞;GVHD;移植成活率 　　Experimental Study of Donor Natural Killer Cells Reducing Graft-versus-host Disease and Enhancing Engraftment 　　AbstractThe purpose of this study was to investigate whether pretransplant infusion of reactive natural killer cells（NK cells）from donor or recipient can reduce graft-versus-host disease（GVHD）and enhance engraftment in bone marrow transplantation（BMT）. Recipient BALB/c mice were divided into 4 groups after received 6.5 Gy total-body irradiation (TBI): control group 1 was treated with nothing, control group 2 received BMT alone, experiment group 1 received BMT and autoreactive NK cells, experiment group 2 received BMT and alloreactive NK cells. Life span, clinical and pathologic changes of GVHD and chimerism rate of each group were evaluated. The results showed that all mice were survival in control group 1. The life span was shorter in experiment group 1 than that in control group 2 （Plt;0.05）and longer in experiment group 2 than that in control group 2（Plt;0.01）. GVHD was higher in score of experiment group 1 than in control group 2 （Plt;0.05）but lower in experiment group 2 than that in control group 2（Plt;0.01）. The donor chimerism rate in both two experiment groups were higher than that in control group 2 （Plt;0.05）, however, the donor chimerism rate was higher in experiment group 2 than that in experiment group 1（Plt;0.01）. It is concluded that pretransplant infusion of alloreactive donor NK cells