依曲替酸对角质形成细胞肝素结合表皮生长因子样生长因子mRNA影.docVIP

依曲替酸对角质形成细胞肝素结合表皮生长因子样生长因子mRNA影 　 作者：罗素菊，彭振辉，郑焱，张路坤，王蔚，王国荣 【摘要】 目的 研究依曲替酸对培养的正常人角质形成细胞(KC)增殖和肝素结合表皮生长因子样生长因子(HBEGF)mRNA表达的影响。方法 0.1和1μmol/L伊曲替酸处理正常人KC 12h后，用噻唑蓝比色法(MTT法)和实时荧光定量逆转录聚合酶链反应(RTPCR)法分别检测KC增殖和HBEGF mRNA表达的改变。结果 依曲替酸处理12h后，可剂量依赖抑制KC的增殖和上调HBEGF mRNA的表达。0.1和1μmol/L伊曲替酸分别使KC增殖抑制10.2%和14.4%，使HBEGF mRNA增加到正常对照组的3.2和7.1倍。与对照组相比，差异均有统计学意义(Plt;0.05)。结论 依曲替酸可剂量依赖上调HBEGF mRNA的表达，其可能参与依曲替酸对KC增殖的抑制作用。 【关键词】 表皮生长因子；角质形成细胞；维甲酸 　　Effect of acitretin in inducing the expression of heparinbinding epidermalgrowthfactorlike growth factor mRNA in normal human keratinocytes 　　ABSTRACT: Objective To investigate the alteration of cell proliferation and heparinbinding epidermalgrowthfactorlike growth factor(HBEGF)mRNA expression in cultured normal human keratinocytes after acitretin treatment. Methods After a 12hour incubation with 0.1 and 1μmol/L acitretin in normal human keratinocytes, the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of HBEGF mRNA was examined by realtime quantitative reverse transcription polymerase chain reaction (RTPCR). Results Both keratinocytes growth inhibition and HBEGF mRNA expression was dosedependently induced by acitretin. Treatment with 0.1 and 1μmol/L acitretin inhibited keratinocyte proliferation by 10.2% and 14.4%, and elevated HBEGF mRNA by 3.2and 7.1fold, respectively. Conclusion Upregulated HBEGF mRNA expression induced by acitretin in normal human keratinocytes may be involved in regulating keratinocyte growth inhibition after acitretin treatment. 　　KEY WORDS: epidermal growth factor; keratinocyte; retinoid 　　维甲酸(retinoic acid, RA)是维生素A的代谢物或衍生物，具有广泛的生物学功能，包括参与调控细胞的增殖、分化和凋亡，抑制黑色素生成，抑制皮脂分泌，调节免疫功能，抗衰老等。自人工合成全反式RA后，RA已被广泛应用于多种皮肤科疾病的治疗，如角化性皮肤病、痤疮、银屑病、皮肤光老化等，并且取得了良好的疗效。通常认为RA通过两类受体发挥作用，即维甲酸受体(retinoic acid receptors, RAR)和维甲酸X受体(retinoid X receptors, RXR)，但其具体作用机制至今尚不完全清楚。体外角质形成细胞(keratinocyte, KC)培养、皮肤器官培养、皮肤活检及动物实验研究表明RA可诱导HBEGF的表达，提示RA可能部分通过调节HBEGF的表达，从而发挥其生物学功能[1]。肝素结合表皮