依达拉奉对兔肺缺血再灌注损伤保护作用.docVIP

依达拉奉对兔肺缺血再灌注损伤保护作用 　【摘要】 　　目的：利用改良兔在体肺保护模型，探讨氧自由基清除剂依达拉奉对在体肺保护的作用及可能机制。方法： 健康家兔20只，随机分为低钾右旋糖酐液（LPD液）组与依达拉奉（LPE液）组，每组10只，分别以LPD液和LPE液进行肺动脉灌洗和保存，每组分别于等时点取左下肺静脉血行血气分析检测静脉血氧分压（PvO2），测定血浆丙二醛（MDA）、超氧化物歧化酶（SOD）、诱导型一氧化氮合酶（iNOS）、内皮型一氧化氮合酶（eNOS），肺组织湿干重比(W/D)，光镜、透射电镜下观察肺组织形态结构变化。结果： 两组动物再灌注后各时点PvO2均较缺血前下降，但LPE液组明显高于LPD液组，再灌注后LPE液组血浆MDA、iNOS表达明显低于LPD液组，SOD、eNOS表达则高于LPD液组，肺组织湿干重比LPE液组明显低于LPD液组，组织形态学检查LPE液组结构损伤变化较LPD液组轻。结论： 加入依达拉奉的LPD液能够减轻肺缺血再灌注损伤，具有较好的肺保护效果。 【关键词】 依达拉奉； 肺缺血再灌注； 保护作用； 兔 　　［Abstract］Objective: To investigate the preservative effect of edaravone on rabbits lung ischemiareperfusion injury.Methods： Twenty rabbits were randomly divided into two groups. In LPD group,the lungs were perfused and preserved with LPD solution,while in LPE group,the lungs were with LPD+Edaravone solution.In reperfusing the blood samples were got at the same time.The PvO2,iNOS,eNOS,SOD and MDA,lung wet/dry weight ratio were detected.Lung samples were examined by pathology. Results： At the same time,the PvO2 was higher in the edaravone group than that in the LPD group.The levels of eNOS and SOD were higher in the edaravone group than that in the LPD group,while the levels of MDA and iNOS were lower in the edaravone than that in LPD group.The wet/dry weight ratio was significantly lower in Edaravone group than that in LPD group. Conclusion： Edaravone could help in clearing of oxygen free radical and result in alleriating of lung ischemiareperfusion injury. Edaravone had positive effect on preservative quality of the ischemiareperfusion lung. 　　［Key words］edaravone; lung ischemiareperfusion; preservative effect; rabbit 随着移植相关各项技术的更新和发展,肺移植已成为治疗终末期肺气肿、晚期肺实质性病变和肺血管病变唯一有效的方法,但手术后早期的肺缺血再灌注损伤仍是患者术后发生肺功能不全导致死亡的重要原因。肺缺血再灌注损伤的重要原因之一就是氧自由基形成。依达拉奉是一种新型的自由基清除剂,对多种脏器及组织的缺血再灌注损伤有保护作用。本研究拟通过建立兔在体肺保存模型,探讨依达拉奉对肺损伤的保护作用及可能机制。 　　1 材料和方法 1.1 动物分组 健康家兔20只，雌雄不限，体质量2 500～3 000 g，由江苏大学实验动物中心提供，随机分为低钾右旋糖酐液（LPD液）组和依达拉奉（LPE液）组，每组10只，分别以LPD液和加入依达拉奉的LPD液进行肺动脉灌洗和保存。LPD液配制成分（Na+ 141 mmol/L，K+ 6.