多负荷因素联合对小鼠上肢肌肉抗脂质过氧化反应影响.docVIP

多负荷因素联合对小鼠上肢肌肉抗脂质过氧化反应影响 　 作者：戴文涛,赵晓蓉,杨翠婵,符古雅,李伯灵,余日安 【摘要】 目的 探讨多负荷因素联合对小鼠上肢肌肉抗脂质过氧化反应的影响。方法 昆明种雄性小鼠54只，随机分9组，对各组上肢肌肉均施以3种负荷因素(固定天数、每日固定时间和固定姿势)、每因素高低两种水平的肌肉负荷后，检测各组上肢肌肉超氧化物歧化酶(SOD)和氧化亚氮合酶(NOS)活性，分析三负荷因素对SOD和NOS活性的影响。结果 固定7 d组SOD活性比正常对照组及14 d组高(Plt;0.05)，所有实验组NOS活性均比正常对照组高(Plt;0.05)。结论 多负荷因素联合作用早期小鼠上肢肌肉抗氧化作用增强。 【关键词】 肌肉骨骼疾患;抗氧化作用;超氧化物歧化酶;氧化亚氮合酶;小鼠 Abstract:Objective To study the effects on antilipid peroxidation in upper limb muscles in mice by different load factors in combination.Methods 54 mice were randomized into 9 groups，and three kinds of load factors for 7 days or 14 days for each factor,i.e.,immobilization days,immobilization duration per day,and forced posture,were loaded to upper limb muscles in mice in each group,and then superoxide dismutase (SOD) activities and nitric oxide synthase (NOS) activities of upper limb muscles of mice in each group were examined,and the effects of the three kinds of load factors on SOD and NOS activities in upper limb muscles of mice were analyzed.Results SOD activity of the upper limb muscles in mice of the 7day immobilization group was higher than those in the control group and 14day immobilization group (Plt;0.05),and NOS activities of the upper limb muscles in mice in all of the test groups were higher than that in the control group (Plt;0.05).Conclusion The antilipid peroxidation of the upper limb muscles in mice increased in the early stage when loaded with different load factors in combination. 　　Key words: musculoskeletal disorders; the antilipid peroxidation; superoxide dismutase; nitric oxide synthase;mice 　 职业性肌肉骨骼疾患(occupational musculoskeletal disorders，OMDs)是严重影响职业生命质量和劳动生产率的职业相关疾病，是发达国家职业病赔偿的主要病种，在发展中国家造成的损失也越来越大[1]。关于OMDs的研究，有助于辨明其发病危险因素，继而针对劳动环境、作业负荷和劳动组织等OMDs危险因素采取有效的干预措施，降低发病率。近年，多因素负荷联合对肌肉应激的影响是OMDs的研究热点[1]。脂质过氧化与抗脂质过氧化反应是肌肉正常的应激过程，也是肌肉应激状态的反映，可以用于研究多负荷因素下肌肉的反应。本实验利用间歇性强迫体位小鼠模型，探讨多负荷因素联合作用对小鼠上肢肌肉抗脂质过氧化的影响。 　　1 材料与方法 　　1.1 实验动物及分组 　　健康SPF级昆明种雄性小鼠54只，体质量(34.0±3.0)g，由广东省医学实验动物中心提供，合格证号：SCXK(粤)20080002