当归六黄汤分煎样品及合煎样品中阿魏酸、盐酸小檗碱、黄芩苷含量.doc

当归六黄汤分煎样品及合煎样品中阿魏酸、盐酸小檗碱、黄芩苷含量 　 作者：郑亚玉，梁光义，钟正灵，张丽艳，靳凤云，曹佩雪，杨玉琴，陈添福 【摘要】 目的对当归六黄汤分煎样品与合煎样品中阿魏酸、盐酸小檗碱、黄芩苷的含量进行比较，从而为其临床应用提供依据。方法均采用反相液相色谱法，DIKMA ODSC18柱(5 μm 4.6 mm×250 mm)，以0.085％磷酸－乙腈为流动相梯度洗脱，流速为1.0 ml·min-1，柱温：35℃，检测波长为326nm测定阿魏酸。以乙腈－0.5%的三乙胺（磷酸调pH=3.00)（27∶73）为流动相，流速为1.0 ml·min-1，柱温：30℃，检测波长为343 nm测定盐酸小檗碱。以甲醇水－磷酸(55∶45∶0.2)为流动相，检测波长为280 nm，流速为1.0ml·min-1，柱温30℃测定黄芩苷。结果3个指标性成分在分煎样品中平均含量均大于合煎样品中平均含量。结论从中药复方汤剂中指标性成分的含量考虑，当归六黄汤配方颗粒应用于临床具有一定的可行性。 【关键词】 当归六黄汤； 阿魏酸； 盐酸小檗碱； 黄芩苷 China　Abstract：ObjectiveTo compare the contents of ferulic acid、berberine 、baicalin in single medicine decoction and mixed medicine decoction of Danguiliuhuang decoction， and to provide a scientific basis of Danguiliuhuangdecoction for clinic. MethodsThe samples were determined by HPLC. DIKMA ODSC18 column (5 μm, 4.6 mm×250 mm)was used， the mobile phase consisted of 0.085% phosphoricacetonitrile with flow rate 1.0ml·min-1 ， The detection wavelength was set at 326 nm and the column temparature was 35℃ and Ferulic Acid was detected. The mobile phase consisted of acetonitrile and water（27:73)，which was adjusted to pH3.0 by 0.5％ triethylamine solution and phosphofic acid with flow rate 1.0 ml·min-1 ， the detection wavelength was seted at 343nm ，the column temparature was 30℃and berberine was detected . The mobile phase consisted of methanolwaterphosphoric （55：45：0.2）with flow rate 1.0 ml·min-1. The detection wavelength was set at 280 nm，the column temprature was 30℃ and baicalin was detected.ResultsThe contents of ferulic acid 、berberine 、baicalin in single medicine decoction was higher than in mixed medicine decoction.ConclusionIt can be used in clinic as a reference. 　　Key words： Dangguiliuhuang decoction；Ferulic acid；Berberine；Baicalin 当归六黄汤出自李东垣《兰室秘藏》，由当归6 g，生地黄6 g，熟地黄6 g，黄连6 g，黄芩6 g，黄柏6 g，黄芪12 g 7味药组成，药材均为粗末。每服五钱（15g），水两盏（300 ml），煎至一盏（150 ml），口服［1］，主治阴虚火旺证。配方颗粒汤剂与传统汤剂的化学成分、药效是否有明显的差异，配方颗粒是否应广泛应用于临床，还存在争论。本文以阿魏酸、盐酸小檗碱、黄芩苷的含量为指标，采用HPLC法比较该方中药配方颗粒（分煎）与传统汤剂（合煎）制得的汤剂中指标性成分的含量是否有差异，从有效成分含量对比方面，为