热休克蛋白27参及兔肺缺血预处理保护.docVIP

热休克蛋白27参及兔肺缺血预处理保护 　 作者：涂军伟，徐正 王万铁，吴成云，方舟溪 【摘要】 目的:探讨热休克蛋白27（HSP27）在肺缺血预处理保护中所起的作用及意义。方法：采用在体兔单肺原位缺血再灌注模型，30只日本大耳兔随机均分为三组：假手术（S）组、缺血/再灌注（I/R）组及缺血预处理（PC）组，实验检测肺组织湿干重比和肺泡损伤数比值，电镜评定肺组织超微结构损伤，免疫组化检测肺组织热休克蛋白27的定位及表达。结果：①I/R组肺组织湿干重比（W/D）及肺泡损伤数比值(IAR)明显于Ｓ组（均Plt;0.01）；PC组W/D及IAR均显著低于I/R组（Plt;0.05或Plt;0.01）。②免疫组化显示PC组肺小动脉内膜及外膜层、肺小静脉壁全层、远端呼吸性细支气管及少许肺泡上皮HSP27有强表达；肺小静脉HSP27含量（平均吸光度值LD）高于I/R组和Ｓ组（均Plt;0.01）。③I/R组肺组织超微结构的损伤明显，而PC组损伤明显减轻。结论 ：肺缺血预处理能减轻肺缺血再灌注损伤，HSP27表达增加并参与肺缺血预处理保护。 【关键词】 热休克蛋白；兔；缺血预处理；缺血再灌注损伤 Abstract: Objective: To investigate the effect and significance of heat shock protein-27 in the protection afforded by lung ischemic preconditioning in rabbits. Methods: Single lung in situ ischemic reperfusion animal model was used. 30 rabbits were randomly divided into three groups: sham (S) group， ischemic/reperfusion (I/R) group and ischemic preconditioning (PC) group. Wet to dry ratio (W/D) and injured alveoli rate (IAR) were determined. Ultramicroscopic structural change was observed under electron microscope. Heat shock protein-27 was detected with immunohistochemistry. Results:①W/D and IAR in I/R group were higher than that in S group （Plt;0.01）. The W/D and IAR in PC group were lower than that in I/R group (Plt;0.05， Plt;0.01 respectively). ②The heat shock protein-27 strongly expressed in intima and extima of small pulmonary artery，vessel wall of small pulmonary vein， respiratory brochiole and part of alveolar epithelium. The light density of heat shock protein-27 in small pulmonary veins in PC group was higher than that of other groups. ③Electron microscope showed serious lung injury occurred in I/R group while there was no significant injury in PC group. Conclusion: Lung ischemic preconditioning can mitigate lung ischemia reperfusion injury，highly expressed heat shock protein-27 participated in the protection afforded by lung ischemic preconditioning. Key words: heat shock protein；rabbit；ischemic preconditioning；ischemia reperfusion injury 热休克蛋白（HSP）是