益气补肾温经法对去卵巢大鼠股骨颈骨形态计量学影响.docVIP

益气补肾温经法对去卵巢大鼠股骨颈骨形态计量学影响 　 作者：吴连国 陈华 史晓林 刘康 徐建春 毛应德龙 朱彦昭 【摘要】 ［目的］观察自拟强骨饮对骨质疏松症的骨形态计量学的影响，并探讨其机制。［方法］健康雌性SD大鼠90只，体重230～280g。随机分为3组，分别为治疗组、对照组和空白组，每组30只。分别采取切除双侧卵巢方法进行骨质疏松造模，10周造模成功后，开始给药，治疗组用自拟强骨饮灌胃，每日一次，每次1ml/g，对照组用密盖息，皮下注射，每日1次，每次0.72u/kg。空白组，不做处理。在给药后3、4.5、6个月每组各取10只大鼠进行检测，先测量体重，空气栓塞处死，分离股骨颈，获得股骨颈样本，经切片等处理后，显微镜下作骨形态计量学检测。［结果］给药后治疗组3个月骨体积分数与密盖息组数据相比有统计学意义，与空白组相比骨小梁厚度、骨小梁间距、骨体积分数都具有统计学意义。给药后4.5个月强骨饮组骨小梁厚度与密盖息组相比差异均有统计学意义。给药后6个月强骨饮组骨小梁厚度、骨小梁间距与密盖息组相比均具有统计学意义。 ［结论］自拟强骨饮可以明显改善骨形态计量学指标，可能是通过刺激成骨细胞生长，抑制破骨细胞活性，并抑制高骨转趋势来实现的。 【关键词】 强骨饮;骨形态计量学;骨质疏松症；益气补肾温经；实验研究 　　Abstract：［Objective］To explore the bone histomorphology effect of QiangGuYin on neck of femur of ovariectomized rats.［Methods］Ninety femal SD rats，weight 230～280 gram，were rangdonmized into three groups: QiangGuYin group（treatment group），Miacalcic group (contrast group) and control group.All rats were variectomized.After 10 weeks，the osteoporosis model succeed，the treatment group，contrast group were given QiangGuYin，Miacalcic respectively.The treatment group used QiangGuYin everyday，1ml/g.The contrast group used Miacalcic 0.72u/kg，everyday hyodermic.And 3，4.5，6 months after the treatment，kill 10 rats to explore.The neck of femur were cut and stined with Masson Golder Trichrome for bone histomorphomemetric contrast group analysis.［Result］The treatment group ovariectomized rats in 3m，BV/TV compared with statistical significance;compared with control group in Tb.sp，BV/TV，Tb.Th with statistical significance.After 4.5m treatment group Tb.Th compared with contrast group have statistical significance.Give the medicine 6m，Tb.sp，Tb.Th compared with contrast group all have statistical significance.［Conclusion］Giangguyin can effectively improve the histomorphometry index.The mechanism probably is stimulating osteogenesis and supperssing the cytoactive of osteoclast and trend of the high bone transform. 　　Key words:Qiangguyin;histomorphometry;ostroporosis；experimental research;TCM therapy 　　骨质疏松症是妇女绝经后常见的一种全身性代谢骨骼疾病，因骨丢失所造成