石油醚提取过50目及过325目筛温莪术粉挥发油收率比较.docVIP

石油醚提取过50目及过325目筛温莪术粉挥发油收率比较 　 作者：张学愈 盛勇 邹俊 涂铭旌 潘年松 【摘要】 　　目的探讨并比较石油醚回流提取温莪术粗粉和微纳米粉体挥发油的最佳工艺。方法将温莪术粉碎为过50目筛的粗粉与过325目筛的微粉，采用L18(21×37)表之正交实验设计，以温莪术挥发油的提油率为考察指标，以粉碎度、提取时间、加石油醚质量倍数及提取次数为工艺考察对象。 结果以温莪术的挥发油提取率为考察指标，由直观分析可知各因素对提取的影响作用大小依次为提取次数＞加石油醚质量倍数＞提取时间＞粉碎度。方差分析结果表明，在石油醚回流提取温莪术挥发油的工艺中，提取次数和加石油醚的质量倍数对提油率有高度显著影响，提取时间和粉碎度有显著影响。提油率为：2.09%～3.62%，以过325目筛温莪术粉体，加20倍量石油醚，回流2 h，提取3次的提油率最高，提油率为3.62%。结论该法的最佳工艺为：过325目筛温莪术微纳米粉体，加20倍质量倍数的石油醚，回流2 h，提取3次。 【关键词】 温莪术； 挥发油； 粒径； 石油醚回流提取； 正交实验 　　Abstract：ObjectiveTo investigate and compare the best extraction method by petroleum ether from Curcuma wenyujin Y.H. Chen et C.Ling powder and micronanometer powder.MethodsCurcuma wenyujin Y.H. Chen et C.Ling was grinded in 50 mesh powder and 325 mesh micronanometer powder, then extracted by circumfluence extraction method of petroleum ether. Conditions for the extraction percentage of volatile fluid were studied by orthogonal experimental design of L18(21×37), influences of degree of grinding, process time of extraction, quality of petroleum ether and times of extraction were investigated. ResultsThe intuitionistic analysis showed the order of effect of times of extractiongt; quality of petroleum ethergt; process time of extractiongt; degree of grinding. Analysis of variance showed that in the process by circumfluence extraction method of petroleum ether, times of extraction,quality of petroleum ether, process time of extraction and degree of grinding had significant effect on the extraction rate. ConclusionThe optimum condition was micronanometer powder of 325 mesh Curcuma wenyujin Y.H. Chen et C.Ling,20 times amount of petroleum ether,refluring for 2 h,and extracting 3 times. 　　Key words：Curcuma wenyujin Y.H. Chen et C.Ling; Volatile fluid; Particle size; Circumfluence extraction method of petroleum ether; Orthogonal experiments 　　温莪术为姜科植物温郁金Curcuma wenyujin Y.H.Chen et C.Ling的干燥根茎，具行气破血，消积止痛之功。用于治疗癥瘕痞块，淤血经闭，食积胀痛，早期宫颈癌［1］。现代研究表明，莪术具有抗肿瘤、抗病毒抗菌、保肝和抗氧化等活性，其中对于莪术抗肿瘤作用的主要成分目前比较一致的认识是挥发油中的有效成分和姜黄素［