肠激安方对腹泻型肠易激综合征模型大鼠T淋巴细胞及5羟色胺影响.doc

肠激安方对腹泻型肠易激综合征模型大鼠T淋巴细胞及5羟色胺影响 　作者：唐洪梅，黄樱华，李得堂,丘振文，阎雪 【摘要】 　　【目的】观察肠激安方对腹泻型肠易激综合征（IBS）模型大鼠T淋巴细胞水平和5羟色胺（5HT）分泌的影响。【方法】选用新生SD大鼠46只，随机分成正常组，模型组，肠激安方低、中、高剂量组（中药低、中、高剂量组，剂量分别为094、188、376g·kg-1·d-1），补脾益肠丸组（剂量为676g·kg-1·d-1）；采用直肠内醋酸刺激法复制肠道高敏感大鼠模型，经大鼠排便统计及肠道敏感性评估确认造模成功后，采用流式细胞术检测各组外周血CD+3、CD+4、CD+8T淋巴细胞亚群水平，采用免疫组化法观察各组大鼠肠组织5HT阳性细胞数和免疫染色强度。【结果】模型组大鼠排便量显著增加，肠道敏感性显著升高，CD+4水平及CD+4/CD+8比值显著降低，CD+8水平显著升高，5HT阳性细胞数及染色强度显著增加（均P＜001）；中药低、中、高剂量组可减少大鼠排便量（治疗第14天），降低肠道敏感性，升高CD+4水平及CD+4/CD+S比值，降低CD+8水平，减少5HT阳性细胞数及染色强度（均P＜001）。【结论】肠激安方治疗IBS的作用可能与改善细胞免疫功能和肠道敏感性，减少5HT分泌有关。 【关键词】 肠激安方/药理学；肠易激综合征/中药疗法；肠易激综合征/免疫学；结肠/病理学；疾病模型，动物；大鼠 　　Abstract: ObjectiveTo observe the effect of Changjian Prescription (CP) on T lymphocytes count and 5hydroxytryptamine (5HT) secretion in rats with diarrhea predominant irritable bowel syndrome (IBS). MethodsFortysix neonatal SD rats were randomized into normal group, model group, low, middle and highdose CP groups (094, 188 and 376g·kg-1·d-1 respectively), and Bupi Yifei Pills group (676g·kg-1·d-1). Continuous internal rectal stimulation with acetate was used to induce the rat model of intestinal hypersensitive IBS. Flow cytometer was used for the quantitative analysis of peripheral levels of T lymphocytes subsets CD+3 , CD+4 and CD+8 , and immunohistochemical method was used to observe 5HTpositive cells number and the immunostaining intensity in the bowels of rats .ResultsIn the model group, the defecation volume and the intestinal sensitivity were increased, CD+4 level and CD+4/CD+8 decreased, CD+8 level increased, 5HTpositive cells number and the immunostaining intensity elevated (P＜001 compared with the normal group). The defecation volume 14 days after treatment and the intestinal sensitivity were decreased, CD+4 level and CD+4/CD+8 increased, CD+8 level decreased, 5HTpositive cells number and the immunostaining intensity lowered in CP groups(P＜001 compared with the model group). ConclusionThe therapeutic mechanism of CP for IBS