a predictive computational model of the kinetic mechanism of stimulus-induced transducer methylation and feedback regulation through chey in archaeal phototaxis and chemotaxis预测计算模型的动力学机制stimulus-induced换能器甲基化和反馈调节通过崔氏在古细菌趋光性和趋化性.pdfVIP

Streif et al. BMC Systems Biology 2010, 4:27 /1752-0509/4/27 RESEARCH ARTICLE Open Access A predictive computational model of the kinetic mechanism of stimulus-induced transducer methylation and feedback regulation through CheY in archaeal phototaxis and chemotaxis 1,2* 2 1* Stefan Streif , Dieter Oesterhelt , Wolfgang Marwan Abstract Background: Photo- and chemotaxis of the archaeon Halobacterium salinarum is based on the control of flagellar motor switching through stimulus-specific methyl-accepting transducer proteins that relay the sensory input signal to a two-component system. Certain members of the transducer family function as receptor proteins by directly sensing specific chemical or physical stimuli. Others interact with specific receptor proteins like the phototaxis photoreceptors sensory rhodopsin I and II, or require specific binding proteins as for example some chemotaxis transducers. Receptor activation by light or a change in receptor occupancy by chemical stimuli results in reversible methylation of glutamate residues of the transducer proteins. Both, methylation and demethylation reactions are involved in sensory adaptation and are modulated by the response regulator CheY. Results: By mathematical modeling we infer the kinetic mechanisms of stimulus-induced transducer methylation and adaptation. The model (deterministic and in the form of ordinary differential equations) correctly predicts experimentally observed transducer demethylation (as detected by released methanol) in response to attractant and repellent stimuli of wildtype cells, a cheY deletion mutant, and a mutant in which the stimulated transducer species is methylation-deficient. Conclusions: We provide a kinetic model for signal processing in photo- and chemotaxis in the ar