antiangiogenic properties of koetjapic acid, a natural triterpene isolated from sandoricum koetjaoe merr抗血管新生koetjapic酸的性质,自然三萜烯隔绝sandoricum koetjaoe稳定.pdfVIP

Nassar et al. Cancer Cell International 2011, 11:12 /content/11/1/12 PRIMARY RESEARCH Open Access Antiangiogenic properties of Koetjapic acid, a natural triterpene isolated from Sandoricum koetjaoe Merr 1 1 1 2 3 Zeyad D Nassar , Abdalrahim FA Aisha , Mohamed BK Ahamed , Zhari Ismail , Khalid M Abu-Salah , Salman A Alrokayan3 and Amin Malik Shah Abdul Majid1,4* Abstract Background: Angiogenesis, the formation of new blood vessels, has become an important target in cancer therapy. Angiogenesis plays an important role in tumor growth and metastasis. Koetjapic acid (KA) is a seco-A-ring oleanene triterpene isolated from S. koetjape. The solvent extract of this plant species was shown previously to have strong antiangiogenic activity; however the active ingredient(s) that conferred the biological activity and the mode of action was not established. Given the high concentration of KA in S. koetjape, an attempt has been made in this study to investigate the antiangiogenic properties of KA. Results: Treatment with 10-50 μg/ml KA resulted in dose dependent inhibition of new blood vessels growth in ex vivo rat aortic ring assay. KA was found to be non-cytotoxic against HUVECs with IC50 40.97 ± 0.37 μg/ml. KA inhibited major angiogenesis process steps, endothelial cell migration and differentiation as well as VEGF expression. Conclusions: The non-cytotoxic compound, KA, may be a potent antiangiogenic agent; its activity may be attributed to inhibition of endothelial cells migration and differentiation as well VEGF suppression. Background tumor will then use the newly formed blood vessels as a Angiogenesis research is the cutting edge technology