antibacterial activity of silver bionanocomposites synthesized by chemical reduction route抗菌活性的银bionanocomposites通过化学还原合成路线.pdfVIP

Bin Ahmad et al. Chemistry Central Journal 2012, 6:101 /content/6/1/101 RESEARCH ARTICLE Open Access Antibacterial activity of silver bionanocomposites synthesized by chemical reduction route Mansor Bin Ahmad1*, Jenn Jye Lim1†, Kamyar Shameli1,2†, Nor Azowa Ibrahim1†, Mei Yen Tay1† and Buong Woei Chieng1† Abstract Background: The aim of this study is to investigate the functions of polymers and size of nanoparticles on the antibacterial activity of silver bionanocomposites (Ag BNCs). In this research, silver nanoparticles (Ag NPs) were incorporated into biodegradable polymers that are chitosan, gelatin and both polymers via chemical reduction method in solvent in order to produce Ag BNCs. Silver nitrate and sodium borohydride were employed as a metal precursor and reducing agent respectively. On the other hand, chitosan and gelatin were added as a polymeric matrix and stabilizer. The antibacterial activity of different sizes of silver nanoparticles was investigated against Gram-positive and Gram-negative bacteria by the disk diffusion method using Mueller-Hinton Agar. Results: The properties of Ag BNCs were studied as a function of the polymer weight ratio in relation to the use of chitosan and gelatin. The morphology of the Ag BNCs films and the distribution of the Ag NPs were also characterized. The diameters of the Ag NPs were measured and their size is less than 20 nm. The antibacterial trait of silver/chitosan/gelatin bionanocomposites was investigated. The silver ions released from the Ag BNCs and their antibacterial activities were scrutinized. The antibacterial activities of the Ag BNC films were examined against Gram-negative bacteria (E. coli and P. aeruginosa) and Gram-positive (S. aureus and M. luteus) by diffusion method using Muller-Hinton agar. Conclusions: The antibacterial activity of Ag NPs