automatic, context-specific generation of gene ontology slims自动的,特定于上下文的一代的基因本体减轻体重.pdfVIP

Davis et al. BMC Bioinformatics 2010, 11:498 /1471-2105/11/498 METHODOLOGY ARTICLE Open Access Automatic, context-specific generation of Gene Ontology slims Melissa J Davis1,2, Muhammad Shoaib B Sehgal1,3, Mark A Ragan1* Abstract Background: The use of ontologies to control vocabulary and structure annotation has added value to genome- scale data, and contributed to the capture and re-use of knowledge across research domains. Gene Ontology (GO) is widely used to capture detailed expert knowledge in genomic-scale datasets and as a consequence has grown to contain many terms, making it unwieldy for many applications. To increase its ease of manipulation and efficiency of use, subsets called GO slims are often created by collapsing terms upward into more general, high- level terms relevant to a particular context. Creation of a GO slim currently requires manipulation and editing of GO by an expert (or community) familiar with both the ontology and the biological context. Decisions about which terms to include are necessarily subjective, and the creation process itself and subsequent curation are time- consuming and largely manual. Results: Here we present an objective framework for generating customised ontology slims for specific annotated datasets, exploiting information latent in the structure of the ontology graph and in the annotation data. This framework combines ontology engineering approaches, and a data-driven algorithm that draws on graph and information theory. We illustrate this method by application to GO, generating GO slims at different information thresholds, characterising their depth of semantics and demonstrating the resulting gains in statistical power. Conclusions: Our GO slim creation pipeline is available for use in conjunction with any GO-annotated dataset, and creates dataset-specific, obje