high sensitivity electrochemical cholesterol sensor utilizing a vertically aligned carbon nanotube electrode with electropolymerized enzyme immobilization胆固醇高灵敏度的电化学传感器利用垂直对齐碳纳米管电极与electropolymerized酶固定化.pdfVIP

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high sensitivity electrochemical cholesterol sensor utilizing a vertically aligned carbon nanotube electrode with electropolymerized enzyme immobilization胆固醇高灵敏度的电化学传感器利用垂直对齐碳纳米管电极与electropolymerized酶固定化.pdf

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high sensitivity electrochemical cholesterol sensor utilizing a vertically aligned carbon nanotube electrode with electropolymerized enzyme immobilization胆固醇高灵敏度的电化学传感器利用垂直对齐碳纳米管电极与electropolymerized酶固定化

Sensors 2009, 9, 8658-8668; doi:10.3390/ OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article High Sensitivity Electrochemical Cholesterol Sensor Utilizing a Vertically Aligned Carbon Nanotube Electrode with Electropolymerized Enzyme Immobilization Anurat Wisitsoraat *, Chanpen Karuwan, Krongkamol Wong-ek, Ditsayut Phokharatkul, Pornpimol Sritongkham and Adisorn Tuantranont Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center, 112 Thailand Science Park, Pahol Yothin Rd., Klong Luang, Pathumthani 12120, Thailand; E-Mails: chanpen.karuwan@nectec.or.th (C.K.); wongek@tu.ac.th (K.W.); ditsayut.phokharatkul@nectec.or.th (D.P.); pornpimol.sritongkham@nectec.or.th (P.S.); adisorn.tuantranont@nectec.or.th (A.T.) * Author to whom correspondence should be addressed; E-Mail: anurat.wisitsoraat@nectec.or.th; Tel.: +662-564-6900; Fax: +662-564-6756. Received: 14 July 2009; in revised form: 30 September 2009 / Accepted: 10 October 2009 / Published: 29 October 2009 Abstract: In this report, a new cholesterol sensor is developed based on a vertically aligned CNT electrode with two-step electrochemical polymerized enzyme immobilization. Vertically aligned CNTs are selectively grown on a 1 mm2 window of gold coated SiO /Si 2 substrate by thermal chemical vapor deposition (CVD) with gravity effect and water-assisted etching. CNTs are then simultaneously functionalized and enzy