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Viruses 2012, 4, 2636-2649; doi:10.3390/v4 112636 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides? Wenji Zhang, Ioana Moldovan, Oleg S. Targoni, Ramu A. Subbramanian 1,* and Paul V. Lehmann Cellular Technology Limited, Shaker Heights, Ohio 44122, USA; E-Mails: Wenji.Zhang@ (W.Z.), Ioana.Moldovan@ (I.M.), Oleg.Targoni@ (O.S.T.), Paul.Lehmann@ (P.V.L.) * Author to whom correspondence should be addressed; E-Mail: Ramu.Subbramanian@ (R.A.S); Tel.: 216-325-7241. Received: 27 September 2012; in revised form: 17 October 2012 / Accepted: 17 October 2012 / Published: 29 October 2012 Abstract: Immune monitoring of T cell responses increasingly relies on the use of peptide pools. Peptides, when restricted by the same HLA allele, and presented from within the same peptide pool, can compete for HLA binding sites. What impact such competition has on functional T cell stimulation, however, is not clear. Using a model peptide pool that is comprised of 32 well- defined viral epitopes from Cytomegalovirus, Epstein-Barr virus, and Influenza viruses (CEF peptide pool), we assessed peptide competition in PBMC from 42 human subjects. The magnitude of the peptide pool-elicited CD8 T cell responses was a mean 79% and a median 77% of the sum of the CD8 T cell responses elicited by the individual peptides. Therefore, while the effect of peptide competition was e