原创力文档non-viral enos gene delivery and transfection with stents for the treatment of restenosis病毒性以挪士基因传递和转染支架再狭窄的治疗.pdfVIP
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non-viral enos gene delivery and transfection with stents for the treatment of restenosis病毒性以挪士基因传递和转染支架再狭窄的治疗
Brito et al. BioMedical Engineering OnLine 2010, 9:56
/content/9/1/56
RESEARCH Open Access
Non-viral eNOS gene delivery and transfection
with stents for the treatment of restenosis
1 1 2 1*
Luis A Brito , Saradha Chandrasekhar , Steven R Little , Mansoor M Amiji
* Correspondence: m.amiji@neu. Abstract
edu
1Department of Pharmaceutical Background: In this study, we have examined local non-viral gene delivery,
Sciences, Northeastern University,
Boston, MA 02115 USA transfection, and therapeutic efficacy of endothelial nitric oxide synthase (eNOS)
encoding plasmid DNA administered using coated stents in a rabbit iliac artery
restenosis model.
Methods: Lipopolyplexes (LPPs) with eNOS expressing plasmid DNA were
immobilized on stainless steel stents using poly(D,L-lactide-co-glycolide) (PLGA) and
type B gelatin coatings. The gene-eluting stents were implanted bilaterally in the
denuded iliac arteries and eNOS transfection and therapeutic efficacy were examined
14 days after implantation.
Results: The results show that non-viral lipopolyplex-coated stents can efficiently
tranfect eNOS locally in the arterial lumen assessed by PCR and ELISA. Human eNOS
ELISA levels were significantly raised 24 hours after transfection compared to controls
(125 pg eNOS compared to 50 pg for all controls including naked DNA). Local
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