perinatal lamb model of respiratory syncytial virus (rsv) infection围产期羔羊呼吸道合胞病毒(rsv)感染模型.pdf

Viruses 2012, 4, 2359-2378; doi:10.3390/v4 102359 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article Perinatal Lamb Model of Respiratory Syncytial Virus (RSV) Infection Rachel J. Derscheid and Mark R. Ackermann * Department of Veterinary Pathology, 2738 College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA; E-Mail: rdersch@ * Author to which correspondence should be addressed; E-Mail: mackerma@; Tel.: +1-515-294-3647; Fax: +1-515-294-5423. Received: 25 September 2012; in revised form: 17 October 2012 / Accepted: 18 October 2012 / Published: 23 October 2012 Abstract: Respiratory syncytial virus (RSV) is the most frequent cause of bronchiolitis in infants and children worldwide. Many animal models are used to study RSV, but most studies investigate disease in adult animals which does not address the unique physiology and immunology that makes infants more susceptible. The perinatal (preterm and term) lamb is a useful model of infant RSV disease as lambs have similar pulmonary structure including airway branching, Clara and type II cells, submucosal glands and Duox/lactoperoxidase (LPO) oxidative system, and prenatal alveologenesis. Lambs can be born preterm (90% gestation) and survive for experimentation although both preterm and term lambs are susceptible to ovine, bovine and human strains of RSV and develop clinical symptoms including fever, tachypnea, and malaise as well as mild to moderate gross and