原创力文档preparation of a polypyrrole-polyvinylsulphonate composite film biosensor for determination of cholesterol based on entrapment of cholesterol oxidase准备polypyrrole-polyvinylsulphonate复合膜生物传感器测定胆固醇基于胆固醇氧化酶的圈套.pdfVIP
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preparation of a polypyrrole-polyvinylsulphonate composite film biosensor for determination of cholesterol based on entrapment of cholesterol oxidase准备polypyrrole-polyvinylsulphonate复合膜生物传感器测定胆固醇基于胆固醇氧化酶的圈套
Sensors 2009, 9, 6435-6445; doi:10.3390/
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Preparation of a Polypyrrole-Polyvinylsulphonate Composite
Film Biosensor for Determination of Cholesterol Based on
Entrapment of Cholesterol Oxidase
Fadime Yıldırımoğlu 1, Fatma Arslan 2, Servet Çete 2,* and Ahmet Yaşar 2
1 Department of Chemistry, Institute of Sciences, Gazi University, Ankara, Turkey
2 Department of Chemistry, Faculty of Arts and Sciences, Gazi University, Ankara, Turkey
* Author to whom correspondence should be addressed; E-Mail: scete@.tr;
Tel.: +90 312 202 11 37; Fax: +90 312 212 22 79
Received: 10 July 2009; in revised form: 3 August 2009 / Accepted: 10 August 2009 /
Published: 20 August 2009
Abstract: In this paper, a novel amperometric cholesterol biosensor with immobilization
of cholesterol oxidase on electrochemically polymerized polypyrrole–polyvinylsulphonate
(PPy–PVS) films has been accomplished via the entrapment technique on the surface of a
platinum electrode. Electropolymerization of pyrrole and polyvinylsulphonate on the Pt
surface was carried out by cyclic voltammetry between -1.0 and +2.0 V (vs. Ag/AgCl) at a
scan rate of 100 mV upon the Pt electrode with an electrochemical cell containing pyrrole
and polyvinylsulphonate. The amperometric determination is based on the electrochemical
detection of H2O2 generated in the enzymatic reaction of cholesterol. Determination of
cholesterol was carried out by the oxidation of enzymatically
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