原创力文档process development for the elucidation of mycotoxin formation in alternaria alternata过程开发的说明在主产霉菌毒素的形成.pdfVIP
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process development for the elucidation of mycotoxin formation in alternaria alternata过程开发的说明在主产霉菌毒素的形成
Brzonkalik et al. AMB Express 2011, 1:27
/content/1/1/27
ORIGINAL Open Access
Process development for the elucidation of
mycotoxin formation in Alternaria alternata
*
Katrin Brzonkalik , Tanja Herrling, Christoph Syldatk and Anke Neumann
Abstract
The black mould Alternaria alternata produces a wide diversity of mycotoxins which are of particular health
concern. Since no maximum allowable limits are set for Alternaria toxins in food and feed, prevention of Alternaria
infestations and mycotoxin spoilage is the only way to avoid health risks. Thus, the understanding of mycotoxin
biosynthesis is essential. For that purpose, a reliable batch process in a 2 L bioreactor was established which
enables the study of several parameters influencing the production of the mycotoxins alternariol (AOH), alternariol
monomethylether (AME) and tenuazonic acid (TA) by A. alternata DSM 12633. Modified Czapek-Dox medium was
used with glucose as carbon source and ammonium and nitrate as nitrogen sources. Consumption of carbon and
nitrogen sources as well as formation of the three mycotoxins were monitored; the average data of five
independent fermentations was plotted and fitted using a logistic equation with four parameters. Maximum
mycotoxin concentrations of 3.49 ± 0.12 mg/L AOH, 1.62 ± 0.14 mg/L AME and 38.28 ± 0.1 mg/L TA were
obtained.
In this system the effect of different aeration rates (0.53 vvm-0.013 vvm) was tested which exerted a great
influence on mycotoxin production. The use of the semi-synthetic Czapek-Dox medium allowed the exchange of
carbon and nitrogen sources for acetate and aspartic acid. The use of acetate instead of glucose resulted in the
sole production of alternariol whereas the exchange of ammonium and nitrate for aspartate enhanced the
production of both AOH and AME whi
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