原创力文档protective effects and potential mechanisms of pien tze huang on cerebral chronic ischemia and hypertensive stroke保护作用和潜在机制的棱角老子黄在脑慢性缺血中风和高血压.pdfVIP
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protective effects and potential mechanisms of pien tze huang on cerebral chronic ischemia and hypertensive stroke保护作用和潜在机制的棱角老子黄在脑慢性缺血中风和高血压
Zhang et al. Chinese Medicine 2010, 5:35
/content/5/1/35
RESEARCH Open Access
Protective effects and potential mechanisms of
Pien Tze Huang on cerebral chronic ischemia and
hypertensive stroke
1,2 1 3 4 1 1 1
Lihong Zhang , Wai Ping Lam , Lanhai Lü , Chunmei Wang , Yeuk Wa Wong , Lok Hang Lam , Hong Chai Tang
1 2 1 4 1 1*
, Maria Sen Mun Wai , Mingwei Wang , Wing Hang Kwong , Sai Ming Ngai , Ying Tat Mak , David Tai Wai Yew
Abstract
Background: Stroke caused by brain ischemia is the third leading cause of adult disability. Active prevention and
early treatment of stroke targeting the causes and risk factors may decrease its incidence, mortality and
subsequent disability. Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-
inflammatory and anti-thrombotic effects that can prevent brain damage. This study aims to investigate the
potential mechanisms of the preventive effects of Pien Tze Huang on brain damage caused by chronic ischemia
and hypertensive stroke in rats.
Methods: The effects of Pien Tze Huang on brain protein expression in spontaneously hypertensive rat (SHR) and
stroke prone SHR (SHRsp) were studied with 2-D gel electrophoresis and mass spectrometric analysis with a matrix-
assisted laser desorption/ionization time-of-flight (MALDI-TOF)/TOF tandem mass spectrometer and on brain cell
death with enzyme link immunosorbent assay (ELISA) and immunostaining.
Results: Pien Tze Huang decreased cell death in hippocampus and cerebellum caused by chronic ischemia and
hypertensive stroke. Immunostaining of caspase-3 resul
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