原创力文档quantitative analysis of two isoflavones in pueraria lobata flowers from eleven chinese provinces using high performance liquid chromatography定量分析两种葛根异黄酮的花朵从中国十一个省份使用高效液相色谱法.pdfVIP
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quantitative analysis of two isoflavones in pueraria lobata flowers from eleven chinese provinces using high performance liquid chromatography定量分析两种葛根异黄酮的花朵从中国十一个省份使用高效液相色谱法
Yao et al. Chinese Medicine 2010, 5:14
/content/5/1/14
R E S E A R C H Open Access
Research
Quantitative analysis of two isoflavones in Pueraria
lobata flowers from eleven Chinese provinces
using high performance liquid chromatography
1 1 2 3 1
Meicun Yao , Yiting Liao , George Q Li , Francis CP Law and Yi Tang*
Abstract
Background: Pueraria lobata flower (Gehua) is a medicinal herb to treat intoxication, hepatic and gastrointestinal tract
lesion induced by alcohol. This study aims to develop a new HPLC method for the determination of two major
isoflavones in P. lobata flowers, namely tectoridin and 6-O-xylosyl-tectoridin.
Methods: A high performance liquid chromatography (HPLC) method with a C18 column (250 mm × 4.6 mm, 5 μm)
was developed for the quantitative analysis of tectoridin and 6-O-xylosyl-tectoridin, the main isoflavone components
in P. lobata flower. A simple gradient of acetonitrile/water (0 min 15:85; 35 min 50:50; 36 min 15:85; 40 min 15:85; v/v)
was used, and 265 nm was selected as detection wavelength. Tectoridin and 6-O-xylosyl-tectoridin were used as the
external standards in quality control of P. lobata flower for the first time. The method was applied to practical use in
quality assessment of eleven batches of P. lobata flower samples in Chinese herbal medicine market.
Results: The peak area response was linear for tectoridin in the 11.8-236.4 μg/mL range with a correlation coefficient of
0.9996 (P 0.001), and for 6-O-xylosyl-tectoridin in the 10.33-185.99 μg/mL range with a correlation coefficient of
0.9984 (P 0.001) respectively. The average recoveries were 102.7-103.7% for tectoridin and 95.7-103.2% for 6-O-
xylosyl-tectoridin (RSDs 3%), and the intra-day and inter-day RSDs of the two components were les
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