quantitative biomarker analysis of synovial gene expression by real-time pcr滑膜基因表达的定量生物标志物分析实时pcr.pdfVIP
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quantitative biomarker analysis of synovial gene expression by real-time pcr滑膜基因表达的定量生物标志物分析实时pcr
Arthritis Research Therapy Vol 5 No 6 Boyle et al.
Research article Open Access
Quantitative biomarker analysis of synovial gene expression by
real-time PCR
1 1 2 1 1
David L Boyle , Sanna Rosengren , William Bugbee , Arthur Kavanaugh and Gary S Firestein
1Center for Innovative Therapy, Division of Rheumatology, Allergy and Immunology, UCSD School of Medicine, La Jolla, California, USA
2Department of Orthopedics, UCSD School of Medicine, La Jolla, California, USA
Correspondence: David L Boyle (e-mail: dboyle@)
Received: 21 Jan 2003 Revisions requested: 22 Apr 2003 Revisions received: 5 Aug 2003 Accepted: 19 Aug 2003 Published: 8 Oct 2003
Arthritis Res Ther 2003, 5:R352-R360 (DOI 10.1186/ar1004)
© 2003 Boyle et al., licensee BioMed Central Ltd (Print ISSN 1478-6354; Online ISSN 1478-6362). This is an Open Access article: verbatim
copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original
URL.
Abstract
Synovial biomarker analysis in rheumatoid arthritis can be used corrected amplification efficiency errors compared with the C(t)
to evaluate drug effect in clinical trials of novel therapeutic method. The inter-assay coefficient of variation was less than
agents. Previous studies of synovial gene expression for these 25% over time. Q-PCR methods were validated by
s
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