quantitative mass spectrometry for bacterial protein toxins — a sensitive, specific, high-throughput tool for detection and diagnosis定量质谱对细菌蛋白质毒素u2014u2014一个敏感的,具体的,高通量检测和诊断的工具.pdfVIP
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quantitative mass spectrometry for bacterial protein toxins — a sensitive, specific, high-throughput tool for detection and diagnosis定量质谱对细菌蛋白质毒素u2014u2014一个敏感的,具体的,高通量检测和诊断的工具
Molecules 2011, 16, 2391-2413; doi:10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Review
Quantitative Mass Spectrometry for Bacterial Protein Toxins —
A Sensitive, Specific, High-Throughput Tool for Detection and
Diagnosis
1 1 2 1
Anne E. Boyer , Maribel Gallegos-Candela , Renato C. Lins , Zsuzsanna Kuklenyik , Adrian
Woolfitt 1, Hercules Moura 1, Suzanne Kalb 1, Conrad P. Quinn 3 and John R. Barr 1,*
1 Centers for Disease Control and Prevention, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA;
E-Mail: aboyer@ (A.E.B.)
2 Battelle Analytical Services, Atlanta, at the Centers for Disease Control and Prevention, 4770
Buford Hwy, NE, Atlanta, GA 30341, USA
3 Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333, USA
* Author to whom correspondence should be addressed; E-Mail: jbarr@;
Tel.: +1-770-488-7848; Fax: +1-770-488-0509.
Received: 14 February 2011; in revised form: 1 March 2011 / Accepted: 9 March 2011 /
Published: 14 March 2011
Abstract: Matrix-assisted laser-desorption time-of-flight (MALDI-TOF) mass
spectrometry (MS) is a valuable high-throughput tool for peptide analysis. Liquid
chromatography electrospray ionization (LC-ESI) tandem-MS provides sensitive and
specific quantification of small molecules and peptides. The high analytic power of MS
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