quantitative phase imaging with scanning holographic microscopy an experimental assesment定量阶段成像扫描全息显微镜实验评价.pdfVIP

BioMedical Engineering OnLine BioMed Central Research Open Access Quantitative phase imaging with scanning holographic microscopy: an experimental assesment Guy Indebetouw*, Yoshitaka Tada and John Leacock Address: Virginia Tech, Physics Department, Blacksburg, VA 24061/0435, USA Email: Guy Indebetouw* - gindebet@; Yoshitaka Tada - ytd@; John Leacock - leacock@ * Corresponding author Published: 28 November 2006 Received: 19 September 2006 Accepted: 28 November 2006 BioMedical Engineering OnLine 2006, 5:63 doi:10.1186/1475-925X-5-63 This article is available from: /content/5/1/63 © 2006 Indebetouw et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract This paper demonstrates experimentally how quantitative phase information can be obtained in scanning holographic microscopy. Scanning holography can operate in both coherent and incoherent modes, simultaneously if desired, with different detector geometries. A spatially integrating detector provides an incoherent hologram of the objects intensity distribution (absorption and/or fluorescence, for example), while a point detector in a conjugate plane of the pupil provides a coherent hologram of the objects complex amplitude, from which