原创力文档quartz crystal microbalance aptasensor for sensitive detection of mercury(ii) based on signal amplification with gold nanoparticles石英晶体微量天平aptasensor敏感检测汞(二)基于信号放大与金纳米粒子.pdfVIP
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quartz crystal microbalance aptasensor for sensitive detection of mercury(ii) based on signal amplification with gold nanoparticles石英晶体微量天平aptasensor敏感检测汞(二)基于信号放大与金纳米粒子
Sensors 2012, 12, 7080-7094; doi:10.3390/s120607080
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Quartz Crystal Microbalance Aptasensor for Sensitive
Detection of Mercury(II) Based on Signal Amplification with
Gold Nanoparticles
Zong-Mu Dong 1 and Guang-Chao Zhao 1,2,*
1 College of Environmental Science and engineering, Anhui Normal University, Wuhu 241000,
China; E-Mail: dzongmu@
2 Anhui Key Laboratory of Chem-Biosensing, School of Chemistry and Materials Science,
Anhui Normal University, Wuhu 241000, China
* Author to whom correspondence should be addressed; E-Mail: gczhao@;
Tel.: +86-55-3591-0721; Fax: +86-55-3591-0720.
Received: 18 April 2012; in revised form: 14 May 2012 / Accepted: 23 May 2012 /
Published: 29 May 2012
Abstract: We show that a short mercury-specific aptamer (MSA) along with gold
nanoparticles (Au-NPs) can be used to determine Hg(II) ion by a combination of
a QCM-based sensor and a flow system. The MSA binds specifically to Hg(II), and the
Au-NPs can amplify the signal to enhance sensitivity. Specifically, the short thiolated
MSAs are immobilized on the surface of the QCM as the capture probe, and the MSAs are
linked to the Au-NPs as the linking probe. The two components can form a sandwich
structure of the T-Hg(II)-T type in the presence of Hg(II) ions. This leads to change in the
mass on the QCM and a change in the resonance frequency. Hg(II) can be determined with
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