regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense rna纤维母细胞生长因子2的表达和细胞周期的监管发展的内生反义rna.pdfVIP

Genes 2012, 3, 505-520; doi:10.3390/genes3030505 OPEN ACCESS genes ISSN 2073-4425 /journal/genes Article Regulation of Fibroblast Growth Factor-2 Expression and Cell Cycle Progression by an Endogenous Antisense RNA Mark Baguma-Nibasheka, Leigh Ann MacFarlane and Paul R. Murphy * Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, 5850 College Street, Halifax, NS B3H 4R2, Canada; E-Mails: mbaguma@ (M.B.-N.); alexandl@dal.ca (L.A.M.) * Author to whom correspondence should be addressed; E-Mail: prmurphy@dal.ca; Tel.: +1-902-494-1579; Fax: +1-902-494-2050. Received: 27 June 2012; in revised form: 31 July 2012 / Accepted: 15 August 2012 / Published: 16 August 2012 Abstract: Basic fibroblast growth factor (FGF2) is a potent wide-spectrum mitogen whose overexpression is associated with immortalization and unregulated cell proliferation in many tumors. The FGF2 gene locus is bi-directionally transcribed to produce FGF2 cis-antisense RNA (NUDT6) from the NUDT6 gene function, while its mRNA has been implicated in the post-transcriptional regulation of FGF2 expression. FGF2 and NUDT6 are co-expressed in rat C6 glioma cells, and ectopic overexpression of NUDT6 suppresses cellular FGF2 accumulation and cell cycle progression. However, the role of the endogenous antisense RNA in regulation of FGF2 is unclear. In the present study, we employed siRNA-mediated gene knockdown to examine the role of the endogenous NUDT6 RNA in regulation of FGF2 exp