rna polymerase activity and specific rna structure are required for efficient hcv replication in cultured cellsrna聚合酶活性和特定的rna结构有效的培养细胞中丙肝病毒复制所必需的.pdfVIP

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rna polymerase activity and specific rna structure are required for efficient hcv replication in cultured cellsrna聚合酶活性和特定的rna结构有效的培养细胞中丙肝病毒复制所必需的.pdf

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rna polymerase activity and specific rna structure are required for efficient hcv replication in cultured cellsrna聚合酶活性和特定的rna结构有效的培养细胞中丙肝病毒复制所必需的

RNA Polymerase Activity and Specific RNA Structure Are Required for Efficient HCV Replication in Cultured Cells 1. 2. 1 1,3 2 1 Asako Murayama , Leiyun Weng , Tomoko Date , Daisuke Akazawa , Xiao Tian , Tetsuro Suzuki , 1 4 5 1 2 Takanobu Kato , Yasuhito Tanaka , Masashi Mizokami , Takaji Wakita *, Tetsuya Toyoda * 1 Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan, 2 Unit of Viral Genome Regulation, Key Laboratory of Molecular Virology Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, People’s Republic of China, 3 Pharmaceutical Research Lab, Toray Industries, Inc., Kanagawa, Japan, 4 Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Aichi, Japan, 5 Research Center for Hepatitis and Immunology, Kohnodai Hospital, International Medical Center of Japan, Chiba, Japan Abstract We have previously reported that the NS3 helicase (N3H) and NS5B-to-3 9X (N5BX) regions are important for the efficient replication of hepatitis C virus (HCV) strain JFH-1 and viral production in HuH-7 cells. In the current study, we investigated the relationships between HCV genome replication, virus production, and the structure of N5BX. We found that the Q377R, A450S, S455N, R517K, and Y561F mutations in the NS5B region resulted in up-regulation of J6CF NS5B polymerase activity in vitro. However, the activation effects of these mutations on viral RNA replication and virus production with JFH-1 N3H appeared to differ. In the presence of the N3H region