rop18 is a rhoptry kinase controlling the intracellular proliferation of toxoplasma gondiirop18是rhoptry激酶控制刚地弓形虫的细胞内增殖.pdfVIP

ROP18 Is a Rhoptry Kinase Controlling the Intracellular Proliferation of Toxoplasma gondii 1 1 2,3 1 2,3 1* Hiba El Hajj , Maryse Lebrun , Stefan T. Arold , Henri Vial , Gilles Labesse , Jean Franc¸ois Dubremetz ´ 1 UMR 5539, Centre National de la Recherche Scientifique, Universite de Montpellier 2, Montpellier, France, 2 Centre de Biochimie Structurale, Centre National de la ´ ´ ´ Recherche Scientifique, Universite Montpellier 1, Montpellier, France, 3 Centre de Biochimie Structurale, Institut National de la Sante et de la Recherche Medicale, Montpellier, France Toxoplasma gondii is an obligate intracellular parasite for which the discharge of apical organelles named rhoptries is a key event in host cell invasion. Among rhoptry proteins, ROP2, which is the prototype of a large protein family, is translocated in the parasitophorous vacuole membrane during invasion. The ROP2 family members are related to protein-kinases, but only some of them are predicted to be catalytically active, and none of the latter has been characterized so far. We show here that ROP18, a member of the ROP2 family, is located in the rhoptries and re- localises at the parasitophorous vacuole membrane during invasion. We demonstrate that a recombinant ROP18 catalytic domain (amino acids 243–539) possesses a protein-kinase activity and phosphorylate parasitic substrates, especially a 70-kDa protein of tachyzoites. Furthermore, we show that overexpression of ROP18 in tr