原创力文档screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey筛选微生物生产cold-active氧化还原酶及其在手性醇氧化的应用。.pdfVIP
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screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey筛选微生物生产cold-active氧化还原酶及其在手性醇氧化的应用。
Mar. Drugs 2011, 9, 889-905; doi:10.3390/md9050889
OPEN ACCESS
Marine Drugs
ISSN 1660-3397
/journal/marinedrugs
Article
Screening of Microorganisms Producing Cold-Active
Oxidoreductases to Be Applied in Enantioselective Alcohol
Oxidation. An Antarctic Survey
1 1 1 2
Lidiane S. Araújo , Edna Kagohara , Thaís P. Garcia , Vivian H. Pellizari and
Leandro H. Andrade 1,*
1 Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, SP 05508-900,
São Paulo, Brazil; E-Mails: lidianepiaui@.br (L.S.A); ednakb@iq.usp.br (E.K.);
thais_pelegrin@.br (T.P.G)
2 Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico 191, SP 05508-120,
São Paulo, Brazil; E-Mail: vivianp@usp.br (V.H.P.)
* Author to whom correspondence should be addressed; E-Mail: leandroh@iq.usp.br;
Tel.: +55-11-3091-2287; Fax: +55-11-3815-5579.
Received: 20 April 2011; in revised form: 17 May 2011 / Accepted: 20 May 2011 /
Published: 24 May 2011
Abstract: Several microorganisms were isolated from soil/sediment samples of Antarctic
Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source
allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated
the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using
derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms,
1
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