temporal gene expression profiling during rat femoral marrow ablation-induced intramembranous bone regeneration时序基因表达分析在大鼠股骨骨髓ablation-induced膜内的骨再生.pdfVIP

Temporal Gene Expression Profiling during Rat Femoral Marrow Ablation-Induced Intramembranous Bone Regeneration 1 1 2 3 3,4 1 Joel K. Wise , Kotaro Sena , Karen Vranizan , Jacob F. Pollock , Kevin E. Healy , W. Frank Hughes , 1 1 D. Rick Sumner , Amarjit S. Virdi * 1 Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, Illinois, United States of America, 2 Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute and Functional Genomics Laboratory, University of California, Berkeley, California, United States of America, 3 Department of Bioengineering, University of California, Berkeley, California, United States of America, 4 Department of Materials Science and Engineering, University of California, Berkeley, California, United States of America Abstract Enhanced understanding of differential gene expression and biological pathways associated with distinct phases of intramembranous bone regeneration following femoral marrow ablation surgery will improve future advancements regarding osseointegration of joint replacement implants, biomaterials design, and bone tissue engineering. A rat femoral marrow ablation model was performed and genome-wide microarray data were obtained from samples at 1, 3, 5, 7, 10, 14, 28, and 56 days post-ablation, with intact bones serving as controls at Day 0. Bayesian model-based clustering produced eight distinct groups amongst 9,062 significant gene probe sets based on similar temporal expression profiles, which were further categorized into three major temporal classes of increased, variable, and decreased expression. Osteoblast