the gtpase activating raprangap domain-like 1 gene is associated with chicken reproductive traitsgtpase激活raprangap域1基因与鸡的生殖特征相关联.pdfVIP

The GTPase Activating Rap/RanGAP Domain-Like 1 Gene Is Associated with Chicken Reproductive Traits 1,2 1 1,3 1,2 1,2 1,2 4 1,2 Xu Shen , Hua Zeng , Liang Xie , Jun He , Jian Li , Xiujuan Xie , Chenglong Luo , Haiping Xu , Min Zhou5, Qinghua Nie1,2, Xiquan Zhang1,2* 1 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China, 2 Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, China, 3 Institute of Animal Science and Veterinary, Hainan Academy of Agricultural Sciences, Haikou, Hainan, China, 4 Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China, 5 Biotechnology Institute, Jiang Xi Education College, Nanchang, Jiangxi, China Abstract Background: Abundant evidence indicates that chicken reproduction is strictly regulated by the hypothalamic-pituitary- gonad (HPG) axis, and the genes included in the HPG axis have been studied extensively. However, the question remains as to whether any other genes outside of the HPG system are involved in regulating chicken reproduction. The present study was aimed to identify, on a genome-wide level, novel genes associated with chicken reproductive traits. Methodology/Principal Finding: Suppressive subtractive hybridization (SSH), genome-wide association study (GWAS), and gene-centric GWAS were used to identify novel genes underlying chicken reproduction. Single marker-trait association analysis with a large population and allelic frequency spectrum analysis were used to confirm the effects of candidate genes. Using two full-sib Ningdu