the identification of unique serum proteins of hiv-1 latently infected long-term non-progressor patients潜伏性感染hiv - 1的独特的血清蛋白质的识别长期non-progressor病人.pdfVIP

Van Duyne et al. AIDS Research and Therapy 2010, 7:21 /content/7/1/21 R E S E A R C H Open Access Research The identification of unique serum proteins of HIV-1 latently infected long-term non-progressor patients 1,2 2 2 2 3 4 Rachel Van Duyne , Irene Guendel , Kylene Kehn-Hall , Rebecca Easley , Zachary Klase , Chenglong Liu , Mary Young4 and Fatah Kashanchi*2,5 Abstract Background: The search for disease biomarkers within human peri pheral fluids has become a favorable approach to preventative therapeutics throughout the past few years. The comparison of normal versus disease states can identify an overexpression or a suppression of critical proteins where illness has directly altered a patients cellular homeostasis. In particular, the analysis of HIV-1 infected serum is an attractive medium with which to identify altered protein expression due to the ease and non-invasive methods of collecting samples as well as the corresponding insight into the in vivo interaction of the virus with infected cells/tissue. The utilization of proteomic techniques to globally identify differentially expressed serum proteins in response to HIV-1 infection is a sign ificant undertaking that is complicated due to the innate protein profile of human serum. Results: Here, the depletion of 12 of the most abundant serum proteins, followed by two-dimensional gel electrophoresis coupled with identification of these proteins using matrix-assisted laser desorption/ionization time-of- flight (MALDI-TOF) mass spectrometry, has allowed for the identification of differentially expressed, low abundant serum proteins. We have analyzed and compared serum samples from HIV-1 infected subjects who are being treated