ultralong c100 mycolic acids support the assignment of segniliparus as a new bacterial genus超长的c100霉菌酸支持segniliparus的分配作为一种新的细菌属.pdfVIP

Ultralong C100 Mycolic Acids Support the Assignment of Segniliparus as a New Bacterial Genus 1. 1. 1 1 1 2 Sunhee Hong , Tan-Yun Cheng , Emilie Layre , Lindsay Sweet , David C. Young , James E. Posey , W. 2 1 Ray Butler *, D. Branch Moody * 1 Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America, 2 Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America Abstract Mycolic acid-producing bacteria isolated from the respiratory tract of human and non-human mammals were recently assigned as a distinct genus, Segniliparus, because they diverge from rhodococci and mycobacteria in genetic and chemical features. Using high accuracy mass spectrometry, we determined the chemical composition of 65 homologous mycolic acids in two Segniliparus species and separately analyzed the three subclasses to measure relative chain length, number and stereochemistry of unsaturations and cyclopropyl groups within each class. Whereas mycobacterial mycolate subclasses are distinguished from one another by R groups on the meromycolate chain, Segniliparus species synthesize solely non- oxygenated a-mycolates with high levels of cis unsaturation. Unexpectedly Segniliparus a-mycolates diverge into three subclasses based on large differences in carbon chain length with one bacterial culture producing mycolates that range from C58 to C100. Both the overall chain length (C100) and the chain length diversity