金银花小鼠血清移行成分指纹图谱探究.docVIP

金银花小鼠血清移行成分指纹图谱探究 　【摘要】 目的以小鼠为研究对象，进行金银花血清药物化学成分研究。方法以正常健康小鼠为研究对象，以高效液相色谱法测定血清药物化学成分图谱。结果血清药物化学成分图谱与金银花药材对照图谱比较，绿原酸经口服后吸收迅速，代谢较快，血药浓度2 h后就下降到不足30 min的20%；6，7号峰成分吸收入血后，代谢较慢，血药浓度维持时间长；9号峰成分吸收入血较慢，代谢也较慢，2 h后血药浓度不下降，反而有所上升； S峰（绿原酸峰），5，9，10，17，18，19号等在金银花药材中含量较高的峰，吸收入血的比例相对较低，而6，7号峰的吸收入血的比例相对较高。结论该文首次应用高效液相色谱法进行金银花小鼠血清药物成分图谱研究，方法稳定可靠，重现性好，能够全面反映金银花经胃给药吸收入血药物成分的变化，为建立金银花的谱效关系模型，确定金银花的药效物质与代谢作用机制奠定基础。 【关键词】 金银花； 血清药物化学； 指纹图谱 　　Abstract：ObjectiveTo study the pharmacochemical ingredients of serum, which taken from mouse administered the extract of flos lonicerae. MethodsTo take healthy mouse as research object, and determine the chromatogram of serum pharmacochemical ingredient by HPLC. ResultsBy comparing the chromatogram of serum with which of extract of flos lonicerae, the result indicated that caffeotannic acid was absorbed and removed quickly in stomach, its blood level went down to 20% of which after half an hour within two hous, ingredients of peak 6 and peak 7 removed slowly after they were absorbed into blood, the blood drug level kept steady for a long period of time; ingredient of peak 9 was absorbed and removed slowly, after administration for two hours, the blood drug level did not go down but went up a little. The ingredients of main peaks of chromatogram of the extract of flos lonicerae such as peak S(caffeotannic acid), peak 5, peak 9, peak 10, peak 17, peak 18, peak 19 were absorbed into blood in a low proportion, but those of peak 6 and peak 7 had a higher proportion. ConclusionThe chromatogram of pharmacochemical ingredient of serum of mouse by HPLC is studied for the first time. The method is stable and dependable, and reproducible. The chromatogram can show all the changes of ingredients of the extract of flos lonicerae before and after they are absorbed into blood, which would base for establishment of the relationship model between fingerprint and pharmacodynamics, for ascertainment of pharmacodynamic substance and metabolism mechanism. 　　Key words：Fl